Detailed information of TA system TA072806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 1019322..1019877
• Replicon: chromosome
• Accession: NZ_CP019596
• Organism: Bifidobacterium breve strain LMC520

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BBL520_RS04440
• Protein ID: WP_077149462.1
• Coordinates: 1019322..1019645 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S2ZDC4
• Locus tag: BBL520_RS04445
• Protein ID: WP_003829112.1
• Coordinates: 1019632..1019877 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBL520_RS04415	1015118..1016383	-	1266	WP_081398398.1	Fic family protein	-
BBL520_RS04425	1016924..1017289	-	366	WP_065474609.1	YccF domain-containing protein	-
BBL520_RS04430	1017527..1018396	+	870	WP_077149460.1	class I SAM-dependent methyltransferase	-
BBL520_RS04435	1018491..1019105	+	615	WP_077149461.1	cupin domain-containing protein	-
BBL520_RS04440	1019322..1019645	-	324	WP_077149462.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BBL520_RS04445	1019632..1019877	-	246	WP_003829112.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BBL520_RS04450	1020051..1021307	+	1257	WP_016463167.1	HipA domain-containing protein	-
BBL520_RS04455	1021572..1021763	+	192	WP_003829118.1	hypothetical protein	-
BBL520_RS04460	1021947..1023218	-	1272	WP_077149463.1	MFS transporter	-
BBL520_RS04465	1023375..1024037	-	663	WP_019728044.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11873.70 Da
• Isoelectric Point: 5.0771

>T72806 WP_077149462.1 NZ_CP019596:c1019645-1019322 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRILGLL

Nucleotide

• Length: 324 bp

>T72806 NZ_CP019596:c1019645-1019322 [Bifidobacterium breve]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGATTCTAGGTTTGTT
GTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9339.43 Da
• Isoelectric Point: 4.5881

>AT72806 WP_003829112.1 NZ_CP019596:c1019877-1019632 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT72806 NZ_CP019596:c1019877-1019632 [Bifidobacterium breve]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A087B6Q6