Detailed information of TA system TA072800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2107052..2107581
• Replicon: chromosome
• Accession: NZ_CP019595
• Organism: Staphylococcus aureus strain GD1677

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BZK09_RS10715
• Protein ID: WP_000621175.1
• Coordinates: 2107052..2107414 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: BZK09_RS10720
• Protein ID: WP_000948331.1
• Coordinates: 2107411..2107581 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZK09_RS10690	2104030..2104800	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
BZK09_RS10695	2104775..2105254	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
BZK09_RS10700	2105256..2105582	-	327	WP_001052491.1	anti-sigma factor antagonist	-
BZK09_RS10705	2105701..2106702	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
BZK09_RS10715	2107052..2107414	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BZK09_RS10720	2107411..2107581	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
BZK09_RS10725	2107666..2108814	-	1149	WP_001281139.1	alanine racemase	-
BZK09_RS10730	2108880..2109239	-	360	WP_000581197.1	holo-ACP synthase	-
BZK09_RS10735	2109243..2109734	-	492	WP_001286980.1	PH domain-containing protein	-
BZK09_RS10740	2109727..2111304	-	1578	WP_001294650.1	PH domain-containing protein	-
BZK09_RS10745	2111297..2111776	-	480	WP_001287081.1	hypothetical protein	-
BZK09_RS10750	2111985..2112545	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T72800 WP_000621175.1 NZ_CP019595:c2107414-2107052 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T72800 NZ_CP019595:c2107414-2107052 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT72800 WP_000948331.1 NZ_CP019595:c2107581-2107411 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT72800 NZ_CP019595:c2107581-2107411 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1