Detailed information of TA system TA072774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2081542..2082071
• Replicon: chromosome
• Accession: NZ_CP019593
• Organism: Staphylococcus aureus strain GD705

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BZK07_RS10680
• Protein ID: WP_000621175.1
• Coordinates: 2081542..2081904 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: BZK07_RS10685
• Protein ID: WP_000948331.1
• Coordinates: 2081901..2082071 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZK07_RS10655	2078521..2079291	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
BZK07_RS10660	2079266..2079745	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
BZK07_RS10665	2079747..2080073	-	327	WP_001052491.1	anti-sigma factor antagonist	-
BZK07_RS10670	2080192..2081193	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
BZK07_RS10680	2081542..2081904	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BZK07_RS10685	2081901..2082071	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
BZK07_RS10690	2082156..2083304	-	1149	WP_001281139.1	alanine racemase	-
BZK07_RS10695	2083370..2083729	-	360	WP_000581197.1	holo-ACP synthase	-
BZK07_RS10700	2083733..2084224	-	492	WP_001286980.1	PH domain-containing protein	-
BZK07_RS10705	2084217..2085794	-	1578	WP_001294650.1	PH domain-containing protein	-
BZK07_RS10710	2085787..2086266	-	480	WP_001287081.1	hypothetical protein	-
BZK07_RS10715	2086475..2087035	-	561	WP_001092419.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T72774 WP_000621175.1 NZ_CP019593:c2081904-2081542 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T72774 NZ_CP019593:c2081904-2081542 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAACAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAGCAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTAAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT72774 WP_000948331.1 NZ_CP019593:c2082071-2081901 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT72774 NZ_CP019593:c2082071-2081901 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1