Detailed information of TA system TA072719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-Xre
• Location: 736257..737189
• Replicon: plasmid pSymB
• Accession: NZ_CP019586
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: SMB554_RS29180
• Protein ID: WP_088896494.1
• Coordinates: 736635..737189 (+)
• Length: 185 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: H0G7T7
• Locus tag: SMB554_RS29175
• Protein ID: WP_003534594.1
• Coordinates: 736257..736628 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMB554_RS29155	732144..732527	+	384	WP_010975573.1	RidA family protein	-
SMB554_RS29160	732544..734532	+	1989	WP_088896492.1	acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha	-
SMB554_RS29165	734529..735398	+	870	WP_088896493.1	hydroxymethylglutaryl-CoA lyase	-
SMB554_RS29170	735395..736180	+	786	WP_014530513.1	crotonase/enoyl-CoA hydratase family protein	-
SMB554_RS29175	736257..736628	+	372	WP_003534594.1	DUF2384 domain-containing protein	Antitoxin
SMB554_RS29180	736635..737189	+	555	WP_088896494.1	RES family NAD+ phosphorylase	Toxin
SMB554_RS29185	737325..738695	-	1371	WP_015008367.1	FAD-binding oxidoreductase	-
SMB554_RS29190	738807..739910	-	1104	WP_088896495.1	sulfate/molybdate ABC transporter ATP-binding protein	-
SMB554_RS29195	739926..740801	-	876	WP_003537156.1	sulfate ABC transporter permease subunit CysW	-
SMB554_RS29200	740791..741657	-	867	WP_010975580.1	sulfate ABC transporter permease subunit CysT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1639840	1639840

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (23-178)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (71-120)

Sequences

Toxin

Protein

• Length: 185 a.a.
• Molecular weight: 20434.31 Da
• Isoelectric Point: 6.2225

>T72719 WP_088896494.1 NZ_CP019586:736635-737189 [Sinorhizobium meliloti]
MRKHIPPASRPDPEPTSPVEPITLWRAFVPRWAHMPLSGAGAARFGGRWNPIGMPAIYAARELSTAWAEYNQGFVQHPAL
IVQLELRGARLADLTDASVLLELGVDEAIHRCEWRDALDKGAVPETHRLQSELLARDYHGVIYPSFMSPGGTCVALWCWN
GAEEPRLDVIDPDGRLPRSPASWV

Nucleotide

• Length: 555 bp

>T72719 NZ_CP019586:736635-737189 [Sinorhizobium meliloti]
ATGAGGAAACACATTCCGCCCGCATCGCGACCCGATCCGGAACCGACGTCGCCGGTCGAGCCGATAACGCTCTGGCGCGC
CTTCGTTCCGCGCTGGGCACATATGCCGCTATCCGGCGCGGGTGCCGCTCGCTTCGGTGGCCGCTGGAACCCGATCGGCA
TGCCTGCGATTTATGCTGCGCGCGAGCTTTCCACTGCCTGGGCTGAATATAATCAGGGTTTCGTGCAGCATCCGGCGCTC
ATCGTGCAGCTCGAGTTGCGCGGGGCGAGGCTCGCGGACCTCACGGATGCCAGCGTTCTTCTGGAGCTTGGTGTGGATGA
GGCGATCCATCGCTGCGAATGGCGTGATGCGCTCGACAAAGGCGCGGTGCCGGAGACCCACAGGCTGCAGTCGGAGCTTC
TGGCGCGCGACTATCACGGGGTGATCTATCCATCCTTCATGTCGCCCGGCGGCACCTGCGTCGCGCTCTGGTGCTGGAAC
GGTGCGGAAGAACCGCGTCTCGACGTCATCGATCCCGATGGCCGGCTGCCGAGATCGCCCGCCTCATGGGTTTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13113.26 Da
• Isoelectric Point: 9.9996

>AT72719 WP_003534594.1 NZ_CP019586:736257-736628 [Sinorhizobium meliloti]
MMAVDFQIPAARFGDDHSPFLSARLVADRLGVTLAELAKLIGVARNTLTAKSGARKVDSALSKVVRILAMASEMAGDEAR
AVIWFKHQPIPGWAGKTAFDLVGEGKADKVLAYLESVRAGVYA

Nucleotide

• Length: 372 bp

>AT72719 NZ_CP019586:736257-736628 [Sinorhizobium meliloti]
ATGATGGCCGTTGATTTCCAGATACCTGCAGCCCGTTTCGGGGACGATCATTCCCCGTTTCTCTCGGCCCGCCTTGTCGC
CGACCGGTTGGGCGTCACGCTCGCCGAACTCGCCAAGCTGATCGGTGTCGCCCGCAATACGCTGACGGCGAAATCCGGCG
CCCGGAAGGTCGACAGCGCCTTGAGCAAGGTCGTCCGCATCCTCGCCATGGCTTCCGAAATGGCGGGCGACGAAGCCCGC
GCCGTCATCTGGTTCAAGCACCAGCCTATCCCCGGCTGGGCCGGCAAGACGGCGTTCGATCTGGTCGGCGAGGGCAAGGC
CGACAAGGTGCTCGCCTATCTCGAATCGGTACGTGCCGGCGTCTACGCTTAA

Structures

Antitoxin

• AlphaFold DB: H0G7T7