Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 428408..429075 Replicon plasmid pSymB
Accession NZ_CP019586
Organism Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)


Gene name vapC Uniprot ID A0A7X2CQG3
Locus tag SMB554_RS27550 Protein ID WP_014527488.1
Coordinates 428408..428851 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92WE1
Locus tag SMB554_RS27555 Protein ID WP_010975287.1
Coordinates 428848..429075 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SMB554_RS27530 423865..426057 + 2193 WP_017264375.1 hydantoinase B/oxoprolinase family protein -
SMB554_RS27535 426061..426558 + 498 WP_010975282.1 acetone carboxylase subunit gamma -
SMB554_RS27540 426555..427385 + 831 WP_027988369.1 SDR family oxidoreductase -
SMB554_RS27545 427454..428392 + 939 WP_017264373.1 choline ABC transporter substrate-binding protein -
SMB554_RS27550 428408..428851 - 444 WP_014527488.1 PIN domain-containing protein Toxin
SMB554_RS27555 428848..429075 - 228 WP_010975287.1 hypothetical protein Antitoxin
SMB554_RS27560 429259..429732 - 474 WP_017264371.1 DUF2269 family protein -
SMB554_RS27565 429732..431021 - 1290 WP_014527486.1 SDR family oxidoreductase -
SMB554_RS27575 431390..432682 + 1293 WP_014527485.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
SMB554_RS27580 432800..433681 + 882 WP_010975291.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - htpB 1..1639840 1639840


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-131)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 15592.86 Da        Isoelectric Point: 6.6378

>T72718 WP_014527488.1 NZ_CP019586:c428851-428408 [Sinorhizobium meliloti]
VTFLLDVNVLIALIDPSHIGHDDAHEWFASIGQTAWATCPITENGVIRIVGNPEYPNSPGSPLPAMEIVAKLRSLPGHVF
WPDDVSLVGSSDIIPSKILTSGQVTDTYLLALAKARGGKLATFDRKLSAAAVTKGNSALHLIATNRS

Download         Length: 444 bp

>T72718 NZ_CP019586:c428851-428408 [Sinorhizobium meliloti]
GTGACTTTCCTGCTCGACGTCAATGTGCTGATTGCCTTGATCGATCCGAGCCATATAGGCCATGATGATGCGCACGAATG
GTTCGCGTCGATTGGTCAGACCGCGTGGGCCACCTGCCCAATTACCGAAAATGGTGTCATCCGGATCGTCGGCAATCCCG
AGTATCCCAATTCTCCCGGCTCACCGTTACCCGCGATGGAGATCGTCGCCAAATTGCGGTCGCTCCCCGGTCATGTTTTC
TGGCCGGACGATGTGAGCCTTGTCGGGTCAAGTGATATCATCCCATCGAAAATCCTGACTTCGGGGCAGGTGACTGACAC
CTATCTGCTTGCGCTCGCCAAGGCGCGTGGGGGCAAGCTGGCGACATTCGATCGCAAGCTGTCGGCAGCGGCGGTCACGA
AGGGCAATTCCGCCTTGCACCTGATCGCGACGAATAGGTCGTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8174.39 Da        Isoelectric Point: 9.1096

>AT72718 WP_010975287.1 NZ_CP019586:c429075-428848 [Sinorhizobium meliloti]
MRTTLAIDDDVLIAAKAMATQQQRSVGEVISELARRSLRRPRSGGERNGIPLLSPRPDAPPVTLEIVNALRDELA

Download         Length: 228 bp

>AT72718 NZ_CP019586:c429075-428848 [Sinorhizobium meliloti]
ATGAGAACGACCTTGGCTATTGACGACGATGTCCTGATCGCCGCAAAGGCAATGGCGACCCAACAGCAGCGGAGCGTCGG
CGAGGTAATTTCCGAACTGGCGAGGCGTTCTCTGCGCCGGCCGCGCAGCGGCGGCGAGCGTAATGGAATTCCTCTCCTGT
CGCCTCGTCCGGACGCACCGCCCGTGACGCTCGAGATCGTCAACGCCCTGCGTGACGAACTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7X2CQG3

Antitoxin

Source ID Structure
AlphaFold DB Q92WE1

References