Detailed information of TA system TA072717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 75127..75661
• Replicon: plasmid pSymB
• Accession: NZ_CP019586
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: SMB554_RS25795
• Protein ID: WP_017276480.1
• Coordinates: 75365..75661 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: H0FXK0
• Locus tag: SMB554_RS25790
• Protein ID: WP_003527850.1
• Coordinates: 75127..75375 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMB554_RS25755	70593..71660	+	1068	WP_010974979.1	ABC transporter substrate-binding protein	-
SMB554_RS25760	71657..72700	+	1044	WP_010974980.1	iron ABC transporter permease	-
SMB554_RS25765	72697..73497	+	801	WP_010974981.1	ABC transporter ATP-binding protein	-
SMB554_RS25770	73501..74295	+	795	WP_013850206.1	methyltransferase domain-containing protein	-
SMB554_RS25775	74379..74504	-	126	Protein_63	IS481 family transposase	-
SMB554_RS25785	74864..75019	+	156	WP_010974985.1	hypothetical protein	-
SMB554_RS25790	75127..75375	+	249	WP_003527850.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
SMB554_RS25795	75365..75661	+	297	WP_017276480.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SMB554_RS25800	75742..76002	-	261	Protein_67	helix-turn-helix domain-containing protein	-
SMB554_RS25805	76583..76981	+	399	WP_010974988.1	hypothetical protein	-
SMB554_RS25810	77148..77333	+	186	WP_010974989.1	hypothetical protein	-
SMB554_RS25815	77337..77783	-	447	WP_014527651.1	DNA-binding protein	-
SMB554_RS25820	78073..78282	+	210	WP_003527838.1	dodecin family protein	-
SMB554_RS25825	78816..80195	+	1380	WP_027988292.1	sodium:alanine symporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..1639840	1639840
	-	flank	IS/Tn	-	-	74379..74570	191

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-91)

Antitoxin

• Antitoxin ParD (3-78)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11445.09 Da
• Isoelectric Point: 10.6593

>T72717 WP_017276480.1 NZ_CP019586:75365-75661 [Sinorhizobium meliloti]
MATERPFRLAPTAKADLRKIWRYTARRWSLEQAETYQDQLYTAFEGLAAGTKKGRNVDVRPGYLKYPAGAHIVYFRDRGD
RIDIIRILHGRVDAQRHL

Nucleotide

• Length: 297 bp

>T72717 NZ_CP019586:75365-75661 [Sinorhizobium meliloti]
ATGGCGACTGAACGGCCTTTCCGGCTCGCTCCTACCGCCAAGGCCGACCTTCGGAAGATATGGCGCTACACGGCTCGCCG
CTGGTCTCTGGAACAGGCGGAGACCTACCAAGACCAGCTTTATACTGCATTTGAGGGACTGGCCGCAGGGACGAAGAAAG
GCCGCAATGTCGATGTGCGGCCGGGTTATCTCAAATACCCGGCAGGAGCGCACATCGTCTATTTTCGGGATCGCGGCGAC
CGGATCGACATCATCCGCATCCTGCATGGCCGTGTGGACGCCCAACGGCATTTGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8888.73 Da
• Isoelectric Point: 4.2176

>AT72717 WP_003527850.1 NZ_CP019586:75127-75375 [Sinorhizobium meliloti]
MAARSTSISLGDHFAGFIDSQVQTGRYGSASDVVRAGLRLLEEHEAKVRALEAALIEGEESGEPEPFDNEAFKAEMRAEY
GD

Nucleotide

• Length: 249 bp

>AT72717 NZ_CP019586:75127-75375 [Sinorhizobium meliloti]
ATGGCTGCTAGAAGCACATCTATTTCCCTTGGCGATCACTTCGCCGGTTTCATCGACAGCCAGGTGCAAACCGGCCGCTA
CGGCTCCGCCAGCGACGTTGTGCGGGCTGGACTTCGGCTTTTGGAAGAACACGAGGCGAAGGTAAGGGCGCTGGAAGCGG
CCTTGATCGAGGGCGAAGAATCCGGCGAGCCAGAGCCATTCGACAACGAAGCGTTCAAAGCCGAAATGCGCGCCGAATAT
GGCGACTGA

Structures

Antitoxin

• AlphaFold DB: H0FXK0