Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1149908..1150488 Replicon plasmid pSymA
Accession NZ_CP019585
Organism Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)


Gene name vapC Uniprot ID Q92XP1
Locus tag SMB554_RS23880 Protein ID WP_010968151.1
Coordinates 1150105..1150488 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID F7XCL0
Locus tag SMB554_RS23875 Protein ID WP_013851145.1
Coordinates 1149908..1150108 (+) Length 67 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SMB554_RS23855 1145346..1146050 - 705 WP_088896140.1 haloacid dehalogenase type II -
SMB554_RS23860 1146141..1147013 + 873 WP_088896141.1 LysR family transcriptional regulator -
SMB554_RS33905 1147064..1147219 - 156 WP_017264742.1 hypothetical protein -
SMB554_RS34205 1147304..1147462 - 159 WP_164827175.1 hypothetical protein -
SMB554_RS23865 1147586..1148626 - 1041 WP_017264740.1 NAD(P)H-binding protein -
SMB554_RS23870 1148715..1149602 + 888 WP_027988874.1 LysR family transcriptional regulator -
SMB554_RS23875 1149908..1150108 + 201 WP_013851145.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
SMB554_RS23880 1150105..1150488 + 384 WP_010968151.1 type II toxin-antitoxin system VapC family toxin Toxin
SMB554_RS23885 1150948..1151496 - 549 WP_088896142.1 TetR/AcrR family transcriptional regulator -
SMB554_RS23890 1151608..1152396 + 789 WP_088896317.1 SDR family oxidoreductase -
SMB554_RS23895 1152451..1153545 + 1095 WP_088896143.1 alkene reductase -
SMB554_RS23900 1153657..1154481 + 825 WP_088896144.1 SDR family NAD(P)-dependent oxidoreductase -
SMB554_RS23905 1154530..1155393 + 864 WP_088896318.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - htpB / htpB 1..1422736 1422736


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-113)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14292.54 Da        Isoelectric Point: 6.9172

>T72716 WP_010968151.1 NZ_CP019585:1150105-1150488 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Download         Length: 384 bp

>T72716 NZ_CP019585:1150105-1150488 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin


Download         Length: 67 a.a.        Molecular weight: 7096.22 Da        Isoelectric Point: 11.1107

>AT72716 WP_013851145.1 NZ_CP019585:1149908-1150108 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Download         Length: 201 bp

>AT72716 NZ_CP019585:1149908-1150108 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGTCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92XP1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W6MID2

References