Detailed information of TA system TA072715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 667837..668522
• Replicon: plasmid pSymA
• Accession: NZ_CP019585
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SMB554_RS21410
• Protein ID: WP_017263896.1
• Coordinates: 667837..668256 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F7XET8
• Locus tag: SMB554_RS21415
• Protein ID: WP_013845417.1
• Coordinates: 668253..668522 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMB554_RS21380	663604..663963	+	360	WP_088896051.1	hypothetical protein	-
SMB554_RS21385	664008..664283	-	276	Protein_621	helix-turn-helix domain-containing protein	-
SMB554_RS21390	664643..666016	-	1374	WP_088895703.1	ISNCY family transposase	-
SMB554_RS21395	666286..666666	-	381	WP_003526626.1	transcriptional regulator	-
SMB554_RS21400	666684..666995	-	312	WP_014528619.1	type II toxin-antitoxin system HigB family toxin	-
SMB554_RS21405	667270..667683	+	414	WP_014528620.1	hypothetical protein	-
SMB554_RS21410	667837..668256	-	420	WP_017263896.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMB554_RS21415	668253..668522	-	270	WP_013845417.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
SMB554_RS34175	668660..669011	+	352	Protein_628	LysR family transcriptional regulator	-
SMB554_RS21425	669123..669998	-	876	Protein_629	LysR family transcriptional regulator	-
SMB554_RS21440	670978..671901	-	924	WP_013845420.1	LysR family transcriptional regulator	-
SMB554_RS21445	672018..672887	+	870	WP_014528623.1	aldo/keto reductase family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1422736	1422736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.00 Da
• Isoelectric Point: 4.8722

>T72715 WP_017263896.1 NZ_CP019585:c668256-667837 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEVGPDAICVSIITAAELRYGCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTPSG

Nucleotide

• Length: 420 bp

>T72715 NZ_CP019585:c668256-667837 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTGTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGGTAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATGGATGCGCCAAGAAGGGAT
CATCGAAACTACTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCCGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTCCTTCGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10203.67 Da
• Isoelectric Point: 4.4798

>AT72715 WP_013845417.1 NZ_CP019585:c668522-668253 [Sinorhizobium meliloti]
MPELRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT72715 NZ_CP019585:c668522-668253 [Sinorhizobium meliloti]
ATGCCCGAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCTAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAGGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCCGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A844A3Y5