Detailed information of TA system TA072714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 305165..305772
• Replicon: plasmid pSymA
• Accession: NZ_CP019585
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: SMB554_RS19540
• Protein ID: WP_017264738.1
• Coordinates: 305518..305772 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A6A7ZSN0
• Locus tag: SMB554_RS19535
• Protein ID: WP_017264737.1
• Coordinates: 305165..305521 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMB554_RS19505	300561..300986	+	426	WP_157738211.1	flavin reductase family protein	-
SMB554_RS19510	301254..302438	-	1185	WP_027988876.1	Fic family protein	-
SMB554_RS19520	302977..303462	-	486	WP_017265310.1	GNAT family N-acetyltransferase	-
SMB554_RS19525	303604..304236	-	633	WP_088895952.1	LysE family translocator	-
SMB554_RS34120	304568..305068	+	501	Protein_300	NUDIX domain-containing protein	-
SMB554_RS19535	305165..305521	-	357	WP_017264737.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
SMB554_RS19540	305518..305772	-	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
SMB554_RS19545	306215..307183	+	969	WP_088896287.1	dienelactone hydrolase family protein	-
SMB554_RS19555	307412..308743	-	1332	WP_050564360.1	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1422736	1422736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T72714 WP_017264738.1 NZ_CP019585:c305772-305518 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T72714 NZ_CP019585:c305772-305518 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13120.78 Da
• Isoelectric Point: 5.5799

>AT72714 WP_017264737.1 NZ_CP019585:c305521-305165 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAAGHFAAARLSA

Nucleotide

• Length: 357 bp

>AT72714 NZ_CP019585:c305521-305165 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGGCCATTTTGCCGCGGCGCGCCTGTCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A6A7ZSN0