Detailed information of TA system TA072712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 67264..67934
• Replicon: plasmid pSymA
• Accession: NZ_CP019585
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SMB554_RS18275
• Protein ID: WP_015242680.1
• Coordinates: 67515..67934 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SMB554_RS18270
• Protein ID: WP_088895864.1
• Coordinates: 67264..67518 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMB554_RS18250	63440..64375	+	936	WP_088895862.1	ABC transporter permease	-
SMB554_RS18255	64381..65202	+	822	WP_027989449.1	ABC transporter permease	-
SMB554_RS18260	65199..66113	+	915	WP_010967054.1	ABC transporter ATP-binding protein	-
SMB554_RS18265	66110..67072	+	963	WP_088895863.1	ATP-binding cassette domain-containing protein	-
SMB554_RS18270	67264..67518	+	255	WP_088895864.1	plasmid stabilization protein	Antitoxin
SMB554_RS18275	67515..67934	+	420	WP_015242680.1	type II toxin-antitoxin system VapC family toxin	Toxin
SMB554_RS18280	67903..68331	-	429	WP_153315244.1	GFA family protein	-
SMB554_RS18285	68733..71267	+	2535	WP_088895865.1	PAS domain S-box protein	-
SMB554_RS18290	71295..71657	+	363	WP_015242677.1	response regulator	-
SMB554_RS18295	71714..72874	-	1161	WP_015242676.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB / htpB	1..1422736	1422736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14761.01 Da
• Isoelectric Point: 6.7266

>T72712 WP_015242680.1 NZ_CP019585:67515-67934 [Sinorhizobium meliloti]
MIVLGTNVVSEAMKPTPDPAVRSWLNEQVAETLYLASVTLAELLFGIGALPDGRRKKALTEMLGGVLELFGDRVLPFDIG
AARHYAELAVTARAAGKGFPTADGYIAAIAASRGFIVATRDSSPFEAAGLTVVNPWHHR

Nucleotide

• Length: 420 bp

>T72712 NZ_CP019585:67515-67934 [Sinorhizobium meliloti]
ATGATTGTCCTCGGTACGAATGTCGTCTCCGAGGCGATGAAACCGACCCCCGATCCGGCCGTGCGCTCCTGGTTGAACGA
GCAGGTCGCTGAAACCTTGTATCTGGCCAGTGTGACCTTGGCCGAACTGCTGTTCGGCATCGGTGCGCTGCCGGACGGCC
GGCGCAAGAAGGCTCTGACCGAAATGCTTGGCGGCGTGCTCGAACTGTTTGGCGATCGCGTATTGCCGTTCGACATCGGA
GCTGCTCGCCATTATGCCGAGCTTGCGGTGACCGCTCGAGCGGCCGGCAAGGGATTTCCGACGGCCGACGGATATATTGC
CGCCATTGCCGCCTCCAGGGGATTTATCGTCGCGACCCGCGATAGCAGCCCGTTCGAAGCGGCTGGACTTACCGTGGTAA
ATCCTTGGCACCATCGATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9539.89 Da
• Isoelectric Point: 7.2073

>AT72712 WP_088895864.1 NZ_CP019585:67264-67518 [Sinorhizobium meliloti]
MAILTVRNVPDEVHRALRVRAAMHGRSTEAEVREILESAVKPEQRVRMGDALAELGRRLGLTNDDFAVFDEVRDKARAQP
VRFE

Nucleotide

• Length: 255 bp

>AT72712 NZ_CP019585:67264-67518 [Sinorhizobium meliloti]
ATGGCGATCCTGACGGTCCGGAATGTGCCCGATGAAGTGCATCGCGCCTTGCGCGTGAGAGCGGCGATGCACGGCCGCAG
CACCGAGGCGGAAGTCCGGGAGATTTTGGAAAGTGCGGTGAAGCCCGAGCAGCGCGTTCGCATGGGCGATGCCCTGGCCG
AACTCGGCCGGCGACTGGGGCTGACCAATGACGATTTCGCCGTGTTCGACGAGGTGCGCGACAAGGCGCGGGCGCAGCCC
GTGAGATTTGAATGA