Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3603526..3604187 Replicon chromosome
Accession NZ_CP019584
Organism Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)


Gene name vapC Uniprot ID Q92KY5
Locus tag SMB554_RS17770 Protein ID WP_010970565.1
Coordinates 3603526..3603930 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92KY4
Locus tag SMB554_RS17775 Protein ID WP_010970566.1
Coordinates 3603927..3604187 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SMB554_RS17755 3599069..3600181 + 1113 WP_003535893.1 3-isopropylmalate dehydrogenase -
SMB554_RS17760 3600310..3601017 + 708 WP_010970563.1 phosphatase PAP2 family protein -
SMB554_RS17765 3601118..3603007 - 1890 WP_014527006.1 MHS family MFS transporter -
SMB554_RS17770 3603526..3603930 - 405 WP_010970565.1 type II toxin-antitoxin system VapC family toxin Toxin
SMB554_RS17775 3603927..3604187 - 261 WP_010970566.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
SMB554_RS17780 3604316..3604486 + 171 WP_010970567.1 hypothetical protein -
SMB554_RS17785 3604600..3605634 + 1035 WP_003531790.1 aspartate-semialdehyde dehydrogenase -
SMB554_RS17790 3605807..3606628 + 822 WP_010970568.1 lytic transglycosylase family protein -
SMB554_RS17800 3606975..3607658 - 684 WP_010970569.1 carbonic anhydrase -
SMB554_RS17805 3607832..3608713 - 882 WP_010970570.1 pyridoxal kinase PdxY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(8-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14507.71 Da        Isoelectric Point: 6.7257

>T72711 WP_010970565.1 NZ_CP019584:c3603930-3603526 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Download         Length: 405 bp

>T72711 NZ_CP019584:c3603930-3603526 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9903.16 Da        Isoelectric Point: 5.1253

>AT72711 WP_010970566.1 NZ_CP019584:c3604187-3603927 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Download         Length: 261 bp

>AT72711 NZ_CP019584:c3604187-3603927 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92KY5

Antitoxin

Source ID Structure
AlphaFold DB Q92KY4

References