Detailed information of TA system TA072703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 647502..648079
• Replicon: chromosome
• Accession: NZ_CP019584
• Organism: Sinorhizobium meliloti strain CCMM B554 (FSM-MA)

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: SMB554_RS03100
• Protein ID: WP_010968707.1
• Coordinates: 647502..647765 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: SMB554_RS03105
• Protein ID: WP_010968708.1
• Coordinates: 647762..648079 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SMB554_RS03060	642831..643271	+	441	WP_010968702.1	DUF2948 family protein	-
SMB554_RS03065	643336..644640	+	1305	WP_088895613.1	histidinol dehydrogenase	-
SMB554_RS03070	644637..645113	+	477	WP_010968704.1	UPF0262 family protein	-
SMB554_RS03075	645127..645606	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
SMB554_RS03080	645764..645982	+	219	WP_004435948.1	translation initiation factor IF-1	-
SMB554_RS03085	646080..646700	+	621	WP_004435951.1	Maf-like protein	-
SMB554_RS03090	646705..646917	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
SMB554_RS03100	647502..647765	+	264	WP_010968707.1	BrnT family toxin	Toxin
SMB554_RS03105	647762..648079	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
SMB554_RS03110	648282..648746	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
SMB554_RS03115	648743..648931	-	189	WP_013844020.1	hypothetical protein	-
SMB554_RS03120	649251..650351	+	1101	WP_014526604.1	HPr kinase	-
SMB554_RS03125	650338..651420	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
SMB554_RS03130	651623..651934	+	312	WP_004435964.1	hypothetical protein	-
SMB554_RS03135	652214..652603	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T72703 WP_010968707.1 NZ_CP019584:647502-647765 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T72703 NZ_CP019584:647502-647765 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT72703 WP_010968708.1 NZ_CP019584:647762-648079 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT72703 NZ_CP019584:647762-648079 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCAAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5