Detailed information of TA system TA072677

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2756887..2757416
• Replicon: chromosome
• Accession: NZ_CP019563
• Organism: Staphylococcus aureus strain SR434

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BZP34_RS14400
• Protein ID: WP_000621177.1
• Coordinates: 2757054..2757416 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: BZP34_RS14395
• Protein ID: WP_000948331.1
• Coordinates: 2756887..2757057 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZP34_RS14365	2751923..2752483	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
BZP34_RS14370	2752692..2753171	+	480	WP_001287078.1	hypothetical protein	-
BZP34_RS14375	2753164..2754747	+	1584	WP_001294627.1	PH domain-containing protein	-
BZP34_RS14380	2754734..2755225	+	492	WP_001205910.1	PH domain-containing protein	-
BZP34_RS14385	2755229..2755588	+	360	WP_000581202.1	holo-ACP synthase	-
BZP34_RS14390	2755654..2756802	+	1149	WP_001281147.1	alanine racemase	-
BZP34_RS14395	2756887..2757057	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
BZP34_RS14400	2757054..2757416	+	363	WP_000621177.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BZP34_RS14410	2757766..2758767	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
BZP34_RS14415	2758886..2759212	+	327	WP_001052491.1	anti-sigma factor antagonist	-
BZP34_RS14420	2759214..2759693	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
BZP34_RS14425	2759668..2760438	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13471.72 Da
• Isoelectric Point: 10.1654

>T72677 WP_000621177.1 NZ_CP019563:2757054-2757416 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNTVAHQKN

Nucleotide

• Length: 363 bp

>T72677 NZ_CP019563:2757054-2757416 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATACAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT72677 WP_000948331.1 NZ_CP019563:2756887-2757057 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT72677 NZ_CP019563:2756887-2757057 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1