Detailed information of TA system TA072644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1845297..1845922
• Replicon: chromosome
• Accession: NZ_CP019560
• Organism: Escherichia coli strain KSC1031

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BZY78_RS09465
• Protein ID: WP_000911330.1
• Coordinates: 1845297..1845695 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: BZY78_RS09470
• Protein ID: WP_000450524.1
• Coordinates: 1845695..1845922 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZY78_RS09445	1841175..1841375	+	201	WP_000383836.1	YpfN family protein	-
BZY78_RS09450	1841485..1842183	-	699	WP_000679812.1	esterase	-
BZY78_RS09455	1842257..1844272	-	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
BZY78_RS09460	1844287..1845150	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
BZY78_RS09465	1845297..1845695	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BZY78_RS09470	1845695..1845922	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BZY78_RS09475	1846076..1846789	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
BZY78_RS09480	1847002..1848036	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
BZY78_RS09485	1848053..1848931	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
BZY78_RS09490	1849077..1849649	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
BZY78_RS09495	1849649..1850119	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T72644 WP_000911330.1 NZ_CP019560:c1845695-1845297 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T72644 NZ_CP019560:c1845695-1845297 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT72644 WP_000450524.1 NZ_CP019560:c1845922-1845695 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT72644 NZ_CP019560:c1845922-1845695 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33