Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 929730..930256 Replicon chromosome
Accession NZ_CP019560
Organism Escherichia coli strain KSC1031

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag BZY78_RS04785 Protein ID WP_000323025.1
Coordinates 929730..930017 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag BZY78_RS04790 Protein ID WP_000534858.1
Coordinates 930017..930256 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BZY78_RS04750 925412..926371 - 960 WP_000592549.1 DUF523 and DUF1722 domain-containing protein -
BZY78_RS04755 926564..927088 + 525 WP_000780584.1 lipocalin family protein -
BZY78_RS04760 927244..927621 - 378 WP_001204787.1 antitermination protein -
BZY78_RS04765 927639..928688 - 1050 WP_001265274.1 DUF968 domain-containing protein -
BZY78_RS04770 928690..928968 - 279 WP_032236628.1 hypothetical protein -
BZY78_RS04775 929035..929286 - 252 WP_000980999.1 hypothetical protein -
BZY78_RS04780 929503..929658 - 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
BZY78_RS04785 929730..930017 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
BZY78_RS04790 930017..930256 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
BZY78_RS04795 930281..930586 + 306 WP_071600497.1 hypothetical protein -
BZY78_RS04800 930789..931121 + 333 WP_001349760.1 FlxA-like family protein -
BZY78_RS04805 931558..932898 - 1341 WP_053265882.1 ISNCY family transposase -
BZY78_RS04815 933435..934394 - 960 WP_001533280.1 SIR2 family protein -
BZY78_RS04820 934811..935233 - 423 WP_001599153.1 DUF977 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 895631..944819 49188


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T72634 WP_000323025.1 NZ_CP019560:c930017-929730 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T72634 NZ_CP019560:c930017-929730 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT72634 WP_000534858.1 NZ_CP019560:c930256-930017 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT72634 NZ_CP019560:c930256-930017 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References