Detailed information of TA system TA072622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1086406..1087151
• Replicon: chromosome
• Accession: NZ_CP019516
• Organism: Xanthomonas oryzae pv. oryzae strain CR1-5

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: BXU04_RS05240
• Protein ID: WP_199285672.1
• Coordinates: 1086822..1087151 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: BXU04_RS05235
• Protein ID: WP_011407754.1
• Coordinates: 1086406..1086825 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXU04_RS05215	1082694..1083020	-	327	Protein_987	hypothetical protein	-
BXU04_RS05220 (BXU04_05625)	1083322..1084557	+	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
BXU04_RS05225 (BXU04_05630)	1084595..1085275	-	681	WP_011257926.1	phosphate phosphatase	-
BXU04_RS05230 (BXU04_05635)	1085386..1086354	-	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
BXU04_RS05235 (BXU04_05640)	1086406..1086825	-	420	WP_011407754.1	macro domain-containing protein	Antitoxin
BXU04_RS05240 (BXU04_05645)	1086822..1087151	-	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
BXU04_RS05245 (BXU04_05650)	1087254..1088573	+	1320	WP_155296575.1	IS701-like element ISXo15 family transposase	-
BXU04_RS05250 (BXU04_05655)	1088617..1088952	-	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
BXU04_RS05255 (BXU04_05660)	1089284..1090210	-	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
BXU04_RS05260 (BXU04_05665)	1090256..1091047	+	792	WP_011407758.1	polysaccharide deacetylase family protein	-
BXU04_RS05270 (BXU04_05675)	1091349..1092020	-	672	WP_011407759.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1083322..1088573	5251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T72622 WP_199285672.1 NZ_CP019516:c1087151-1086822 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T72622 NZ_CP019516:c1087151-1086822 [Xanthomonas oryzae pv. oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT72622 WP_011407754.1 NZ_CP019516:c1086825-1086406 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT72622 NZ_CP019516:c1086825-1086406 [Xanthomonas oryzae pv. oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA