Detailed information of TA system TA072585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2985198..2985769
• Replicon: chromosome
• Accession: NZ_CP019512
• Organism: Enterococcus faecalis strain CLB21560

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BZG32_RS15560
• Protein ID: WP_002354774.1
• Coordinates: 2985198..2985539 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: BZG32_RS15565
• Protein ID: WP_002354773.1
• Coordinates: 2985539..2985769 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZG32_RS15550	2981213..2984827	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
BZG32_RS15560	2985198..2985539	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
BZG32_RS15565	2985539..2985769	-	231	WP_002354773.1	hypothetical protein	Antitoxin
BZG32_RS15575	2986176..2986391	-	216	WP_002358974.1	zinc ribbon domain-containing protein	-
BZG32_RS15580	2986530..2987522	+	993	WP_002358973.1	biotin--[acetyl-CoA-carboxylase] ligase	-
BZG32_RS15585	2987589..2988221	-	633	WP_002358972.1	RloB family protein	-
BZG32_RS15590	2988230..2989525	-	1296	WP_002396787.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T72585 WP_002354774.1 NZ_CP019512:c2985539-2985198 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T72585 NZ_CP019512:c2985539-2985198 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAGTCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT72585 WP_002354773.1 NZ_CP019512:c2985769-2985539 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT72585 NZ_CP019512:c2985769-2985539 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812