Detailed information of TA system TA072581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 415753..416249
• Replicon: chromosome
• Accession: NZ_CP019512
• Organism: Enterococcus faecalis strain CLB21560

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R3GZA4
• Locus tag: BZG32_RS02230
• Protein ID: WP_002358470.1
• Coordinates: 415980..416249 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R3JPM7
• Locus tag: BZG32_RS02225
• Protein ID: WP_002413218.1
• Coordinates: 415753..415980 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZG32_RS02200	411897..412427	+	531	WP_002358466.1	hypothetical protein	-
BZG32_RS02205	412442..413266	+	825	WP_002358467.1	hypothetical protein	-
BZG32_RS02210	413642..413905	+	264	WP_002358468.1	hypothetical protein	-
BZG32_RS02215	413898..414455	+	558	WP_002358242.1	hypothetical protein	-
BZG32_RS02220	414458..415693	+	1236	WP_002358469.1	hypothetical protein	-
BZG32_RS02225	415753..415980	+	228	WP_002413218.1	antitoxin	Antitoxin
BZG32_RS02230	415980..416249	+	270	WP_002358470.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BZG32_RS02235	416316..417896	+	1581	WP_002404807.1	DUF3991 domain-containing protein	-
BZG32_RS02240	417913..418617	+	705	WP_010706734.1	hypothetical protein	-
BZG32_RS17205	418643..419062	+	420	WP_025186145.1	TnpV protein	-
BZG32_RS17210	419152..419463	+	312	WP_025186146.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10610.17 Da
• Isoelectric Point: 10.5395

>T72581 WP_002358470.1 NZ_CP019512:415980-416249 [Enterococcus faecalis]
MSYRLEFTPDVKKQLKKMDKYQATLLTRWLYRNIDGTANPREHGKALSANRAGQWRYRIGNYRVIVEIEDERLVVTAIQV
GHRRNVYDK

Nucleotide

• Length: 270 bp

>T72581 NZ_CP019512:415980-416249 [Enterococcus faecalis]
ATGTCCTATCGATTAGAATTTACTCCTGATGTAAAAAAACAGTTAAAAAAAATGGATAAATATCAAGCGACATTGCTAAC
AAGATGGTTGTATCGAAATATTGATGGAACGGCCAATCCTAGAGAGCATGGAAAAGCTTTATCAGCAAATCGTGCAGGTC
AGTGGCGATATAGGATAGGGAATTATCGTGTCATTGTAGAAATAGAAGATGAACGTCTTGTTGTTACAGCCATTCAGGTT
GGCCACAGAAGAAATGTTTACGACAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8785.90 Da
• Isoelectric Point: 4.0179

>AT72581 WP_002413218.1 NZ_CP019512:415753-415980 [Enterococcus faecalis]
MAMITVRVSDEEKEWLSYMAEFFGVTLSDLLKSYSMEQLEDEYDKQTAEIAHKRWIEQGKETISMDEILNEFGGL

Nucleotide

• Length: 228 bp

>AT72581 NZ_CP019512:415753-415980 [Enterococcus faecalis]
ATGGCTATGATTACTGTAAGAGTGAGTGATGAAGAAAAAGAATGGCTTTCTTACATGGCAGAATTTTTCGGTGTGACGTT
GTCGGATTTGTTAAAAAGCTATTCAATGGAACAATTAGAAGATGAGTACGATAAACAAACAGCAGAAATTGCTCACAAAC
GATGGATTGAGCAAGGAAAAGAAACAATTTCTATGGACGAAATATTAAATGAGTTTGGTGGATTATAA

Structures

Toxin

• AlphaFold DB: R3GZA4

Antitoxin

• AlphaFold DB: R3JPM7