Detailed information of TA system TA072559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2890301..2890974
• Replicon: chromosome
• Accession: NZ_CP019488
• Organism: Sinorhizobium meliloti strain B399

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWO90_RS28275
• Protein ID: WP_010969926.1
• Coordinates: 2890570..2890974 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0GA10
• Locus tag: BWO90_RS28270
• Protein ID: WP_003536345.1
• Coordinates: 2890301..2890573 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO90_RS28250	2886292..2887932	-	1641	WP_003536336.1	ABC transporter substrate-binding protein	-
BWO90_RS28255	2887980..2889035	-	1056	WP_010969924.1	dipeptidase	-
BWO90_RS28260	2889215..2889556	-	342	WP_003536339.1	EamA family transporter	-
BWO90_RS28265	2889604..2890164	-	561	WP_010969925.1	TetR/AcrR family transcriptional regulator	-
BWO90_RS28270	2890301..2890573	+	273	WP_003536345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BWO90_RS28275	2890570..2890974	+	405	WP_010969926.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWO90_RS28280	2891102..2891494	+	393	WP_003536349.1	globin	-
BWO90_RS28285	2891500..2891874	+	375	WP_010969927.1	DUF423 domain-containing protein	-
BWO90_RS28290	2891997..2892257	+	261	WP_010969928.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BWO90_RS28295	2892372..2892686	-	315	WP_003536354.1	antibiotic biosynthesis monooxygenase	-
BWO90_RS28300	2892699..2892965	-	267	WP_010969929.1	hypothetical protein	-
BWO90_RS28305	2893018..2893431	-	414	WP_003536357.1	DUF2325 domain-containing protein	-
BWO90_RS28310	2893555..2894481	-	927	WP_010969930.1	energy transducer TonB	-
BWO90_RS28315	2894522..2894956	-	435	WP_010969931.1	hypothetical protein	-
BWO90_RS28320	2895186..2895326	+	141	WP_010969932.1	hemin uptake protein HemP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14747.98 Da
• Isoelectric Point: 5.1974

>T72559 WP_010969926.1 NZ_CP019488:2890570-2890974 [Sinorhizobium meliloti]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T72559 NZ_CP019488:2890570-2890974 [Sinorhizobium meliloti]
TTGAACGGCTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10334.02 Da
• Isoelectric Point: 6.3950

>AT72559 WP_003536345.1 NZ_CP019488:2890301-2890573 [Sinorhizobium meliloti]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT72559 NZ_CP019488:2890301-2890573 [Sinorhizobium meliloti]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Structures

Antitoxin

• AlphaFold DB: H0GA10