Detailed information of TA system TA072558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2479543..2480197
• Replicon: chromosome
• Accession: NZ_CP019488
• Organism: Sinorhizobium meliloti strain B399

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: BWO90_RS26285
• Protein ID: WP_003530010.1
• Coordinates: 2479543..2479731 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: BWO90_RS26290
• Protein ID: WP_010969659.1
• Coordinates: 2479736..2480197 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO90_RS26270	2476109..2477419	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
BWO90_RS26275	2477444..2478415	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
BWO90_RS26280	2478596..2479366	-	771	WP_010969658.1	hypothetical protein	-
BWO90_RS26285	2479543..2479731	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
BWO90_RS26290	2479736..2480197	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BWO90_RS26300	2480516..2481433	-	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
BWO90_RS26305	2481610..2482359	-	750	WP_010969660.1	NUDIX hydrolase	-
BWO90_RS26310	2482362..2483468	-	1107	WP_003530001.1	endonuclease	-
BWO90_RS26315	2483619..2484062	-	444	WP_003529999.1	TIGR02594 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T72558 WP_003530010.1 NZ_CP019488:2479543-2479731 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T72558 NZ_CP019488:2479543-2479731 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT72558 WP_010969659.1 NZ_CP019488:2479736-2480197 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT72558 NZ_CP019488:2479736-2480197 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0