Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-RHH
Location 1677887..1678461 Replicon chromosome
Accession NZ_CP019488
Organism Sinorhizobium meliloti strain B399

Toxin (Protein)


Gene name vapC Uniprot ID Q92QN4
Locus tag BWO90_RS22175 Protein ID WP_010969154.1
Coordinates 1677887..1678252 (-) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92QN3
Locus tag BWO90_RS22180 Protein ID WP_010969155.1
Coordinates 1678246..1678461 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWO90_RS22150 1673027..1674469 + 1443 WP_010969150.1 NADH-quinone oxidoreductase subunit NuoN -
BWO90_RS22155 1674466..1675233 + 768 WP_003531861.1 biotin--[acetyl-CoA-carboxylase] ligase -
BWO90_RS22160 1675261..1676928 + 1668 WP_010969151.1 ribonuclease J -
BWO90_RS22165 1676987..1677391 + 405 WP_010969152.1 methylmalonyl-CoA epimerase -
BWO90_RS22170 1677531..1677800 + 270 WP_014526683.1 DUF1467 family protein -
BWO90_RS22175 1677887..1678252 - 366 WP_010969154.1 type II toxin-antitoxin system VapC family toxin Toxin
BWO90_RS22180 1678246..1678461 - 216 WP_010969155.1 ribbon-helix-helix protein, CopG family Antitoxin
BWO90_RS22185 1678529..1678756 - 228 WP_017264047.1 hypothetical protein -
BWO90_RS22190 1679172..1680500 + 1329 WP_003531873.1 proline--tRNA ligase -
BWO90_RS22195 1680521..1681831 + 1311 WP_014529722.1 lipoprotein-releasing ABC transporter permease subunit -
BWO90_RS22200 1681849..1682535 + 687 WP_010969157.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-40)


Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13592.57 Da        Isoelectric Point: 5.1787

>T72557 WP_010969154.1 NZ_CP019488:c1678252-1677887 [Sinorhizobium meliloti]
MVGALFDTNILIDHLNAVPQAHKELDRFENRAISIITWMEVMVGADAELVEPTRRFLDGFETIALNDEIANRAVTLRRAH
RIKLPDAVIWATAQTAGRLLVTRNTKDFPADDPGIREPYAV

Download         Length: 366 bp

>T72557 NZ_CP019488:c1678252-1677887 [Sinorhizobium meliloti]
ATGGTAGGCGCACTGTTCGATACCAATATCCTTATCGATCATCTGAATGCGGTGCCTCAGGCGCACAAGGAACTTGACCG
TTTCGAAAACCGGGCCATCAGCATCATCACCTGGATGGAGGTGATGGTTGGAGCGGACGCCGAACTGGTTGAGCCGACCC
GGCGCTTCCTCGACGGTTTCGAAACGATCGCCTTGAACGATGAGATCGCCAACCGTGCGGTGACATTGCGCAGGGCGCAC
CGCATCAAGCTACCCGACGCGGTGATCTGGGCAACGGCGCAGACTGCCGGTCGTCTGCTCGTGACGCGCAATACCAAGGA
CTTTCCAGCCGACGACCCGGGGATACGCGAACCCTACGCCGTGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8381.51 Da        Isoelectric Point: 8.6052

>AT72557 WP_010969155.1 NZ_CP019488:c1678461-1678246 [Sinorhizobium meliloti]
MRALIDMNDTQVEALDTLAKRVRRSRAALIREAIDDYLNRHHREQIEDGFGLWGKRKVDGLAYQEKVRGEW

Download         Length: 216 bp

>AT72557 NZ_CP019488:c1678461-1678246 [Sinorhizobium meliloti]
ATGCGAGCCTTGATCGATATGAACGATACCCAGGTCGAAGCCCTAGATACCCTCGCCAAGCGGGTGCGCCGGTCCCGTGC
GGCTCTGATCCGCGAAGCGATCGACGACTATCTCAACCGTCATCATCGCGAGCAGATCGAAGATGGCTTCGGTTTGTGGG
GCAAGCGCAAAGTTGACGGTCTGGCTTATCAGGAGAAGGTGCGCGGCGAATGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92QN4

Antitoxin

Source ID Structure
AlphaFold DB Q92QN3

References