Detailed information of TA system TA072554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 880594..881234
• Replicon: chromosome
• Accession: NZ_CP019488
• Organism: Sinorhizobium meliloti strain B399

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWO90_RS18170
• Protein ID: WP_095919927.1
• Coordinates: 880594..880980 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G6N0
• Locus tag: BWO90_RS18175
• Protein ID: WP_003533689.1
• Coordinates: 880980..881234 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO90_RS18145	876058..876537	+	480	WP_003537307.1	GNAT family N-acetyltransferase	-
BWO90_RS18150	876581..877030	-	450	WP_003537305.1	nucleoside deaminase	-
BWO90_RS18155	877105..878826	+	1722	WP_010968830.1	pseudouridine synthase	-
BWO90_RS18160	878833..879393	+	561	WP_010968831.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BWO90_RS18165	879466..880365	+	900	WP_010968832.1	patatin-like phospholipase family protein	-
BWO90_RS18170	880594..880980	-	387	WP_095919927.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWO90_RS18175	880980..881234	-	255	WP_003533689.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BWO90_RS18180	881373..883205	-	1833	WP_010968833.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
BWO90_RS18185	883357..884703	+	1347	WP_010968834.1	TldD/PmbA family protein	-
BWO90_RS18190	884812..885630	+	819	WP_010968835.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
BWO90_RS18195	885691..885927	+	237	WP_003533684.1	DUF4170 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13846.73 Da
• Isoelectric Point: 4.7010

>T72554 WP_095919927.1 NZ_CP019488:c880980-880594 [Sinorhizobium meliloti]
MVIDTSAIAAIAFNEQEAGSFREKIADDPVRLISAATALEAAMVIETRLGEAAGAELDLWLYKANVEIVAVTAEHMDQAR
RAWRRFGKGRHPAGLNFGDCFSYALAFLTNEPLLFKGSGFSQTDIPAA

Nucleotide

• Length: 387 bp

>T72554 NZ_CP019488:c880980-880594 [Sinorhizobium meliloti]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCGGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTCTTCAAGGGAAGCGGTTTCTCGCAAACCGATATCCCGGCGGCATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9472.63 Da
• Isoelectric Point: 4.8660

>AT72554 WP_003533689.1 NZ_CP019488:c881234-880980 [Sinorhizobium meliloti]
MALSIKDTETEQLARTLARRTGESITLATKRALEERLRRTDTAARRAALLEDLEAIQRRWNAMPVLDSRSSDDIIGYDED
GLPS

Nucleotide

• Length: 255 bp

>AT72554 NZ_CP019488:c881234-880980 [Sinorhizobium meliloti]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGCACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AAGCCATTCAACGCCGCTGGAATGCCATGCCCGTCCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Antitoxin

• AlphaFold DB: H0G6N0