Detailed information of TA system TA072553

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 681857..682434
• Replicon: chromosome
• Accession: NZ_CP019488
• Organism: Sinorhizobium meliloti strain B399

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q92S20
• Locus tag: BWO90_RS17165
• Protein ID: WP_010968707.1
• Coordinates: 681857..682120 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q92KN5
• Locus tag: BWO90_RS17170
• Protein ID: WP_010968708.1
• Coordinates: 682117..682434 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO90_RS17125	677186..677626	+	441	WP_010968702.1	DUF2948 family protein	-
BWO90_RS17130	677691..678995	+	1305	WP_010968703.1	histidinol dehydrogenase	-
BWO90_RS17135	678992..679468	+	477	WP_010968704.1	UPF0262 family protein	-
BWO90_RS17140	679482..679961	+	480	WP_010968705.1	low molecular weight phosphatase family protein	-
BWO90_RS17145	680119..680337	+	219	WP_004435948.1	translation initiation factor IF-1	-
BWO90_RS17150	680435..681055	+	621	WP_004435951.1	Maf-like protein	-
BWO90_RS17155	681060..681272	+	213	WP_010968706.1	DNA gyrase inhibitor YacG	-
BWO90_RS17165	681857..682120	+	264	WP_010968707.1	BrnT family toxin	Toxin
BWO90_RS17170	682117..682434	+	318	WP_010968708.1	BrnA antitoxin family protein	Antitoxin
BWO90_RS17175	682637..683101	-	465	WP_013844019.1	type II toxin-antitoxin system HicB family antitoxin	-
BWO90_RS17180	683098..683286	-	189	WP_013844020.1	hypothetical protein	-
BWO90_RS17185	683606..684706	+	1101	WP_010968710.1	hypothetical protein	-
BWO90_RS17190	684693..685775	+	1083	WP_010968711.1	nucleotidyltransferase family protein	-
BWO90_RS17195	685978..686289	+	312	WP_004435964.1	hypothetical protein	-
BWO90_RS17200	686569..686958	-	390	WP_013844021.1	DUF930 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-82)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (26-103)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9821.16 Da
• Isoelectric Point: 10.3413

>T72553 WP_010968707.1 NZ_CP019488:681857-682120 [Sinorhizobium meliloti]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Nucleotide

• Length: 264 bp

>T72553 NZ_CP019488:681857-682120 [Sinorhizobium meliloti]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12197.83 Da
• Isoelectric Point: 10.0883

>AT72553 WP_010968708.1 NZ_CP019488:682117-682434 [Sinorhizobium meliloti]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Nucleotide

• Length: 318 bp

>AT72553 NZ_CP019488:682117-682434 [Sinorhizobium meliloti]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCGAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Structures

Toxin

• AlphaFold DB: Q92S20

Antitoxin

• AlphaFold DB: Q92KN5