Detailed information of TA system TA072549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 940095..940702
• Replicon: plasmid pSymA
• Accession: NZ_CP019486
• Organism: Sinorhizobium meliloti strain B399

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BWO90_RS05215
• Protein ID: WP_017264738.1
• Coordinates: 940095..940349 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BWO90_RS05220
• Protein ID: WP_040120685.1
• Coordinates: 940346..940702 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO90_RS05195	935496..936200	-	705	WP_014527995.1	haloacid dehalogenase type II	-
BWO90_RS05200	936291..937163	+	873	WP_010968043.1	LysR family transcriptional regulator	-
BWO90_RS32560	937214..937369	-	156	WP_153320124.1	hypothetical protein	-
BWO90_RS32940	937455..937613	-	159	WP_017276730.1	hypothetical protein	-
BWO90_RS05205	937737..938777	-	1041	WP_040120687.1	NAD(P)H-binding protein	-
BWO90_RS05210	938866..939753	+	888	WP_040120686.1	LysR family transcriptional regulator	-
BWO90_RS05215	940095..940349	+	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
BWO90_RS05220	940346..940702	+	357	WP_040120685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BWO90_RS05225	941074..941730	-	657	WP_010968046.1	SGNH/GDSL hydrolase family protein	-
BWO90_RS05230	941760..942710	-	951	WP_010968047.1	ABC transporter permease	-
BWO90_RS05235	942713..943684	-	972	WP_040120683.1	ABC transporter permease	-
BWO90_RS05240	943701..945194	-	1494	WP_010968049.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1117190	1117190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T72549 WP_017264738.1 NZ_CP019486:940095-940349 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T72549 NZ_CP019486:940095-940349 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13178.82 Da
• Isoelectric Point: 5.2288

>AT72549 WP_040120685.1 NZ_CP019486:940346-940702 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAAGHFAEARLSA

Nucleotide

• Length: 357 bp

>AT72549 NZ_CP019486:940346-940702 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGGCCATTTTGCCGAGGCGCGCCTGTCGGCGTAG