Detailed information of TA system TA072547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 215387..215985
• Replicon: plasmid pSymA
• Accession: NZ_CP019486
• Organism: Sinorhizobium meliloti strain B399

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: BWO90_RS01245
• Protein ID: WP_010967243.1
• Coordinates: 215692..215985 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: BWO90_RS01240
• Protein ID: WP_010967242.1
• Coordinates: 215387..215695 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO90_RS01210	211225..211622	-	398	Protein_220	hypothetical protein	-
BWO90_RS01220	212183..212458	+	276	WP_040120625.1	hypothetical protein	-
BWO90_RS01235	214000..215202	+	1203	WP_010967204.1	IS256-like element ISRm3 family transposase	-
BWO90_RS01240	215387..215695	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BWO90_RS01245	215692..215985	+	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
BWO90_RS01250	216174..217508	+	1335	WP_010970069.1	ISNCY-like element ISRm17 family transposase	-
BWO90_RS01255	217884..219083	-	1200	WP_164828840.1	NAD-dependent formate dehydrogenase	-
BWO90_RS01260	219459..220249	+	791	Protein_227	ParB N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1117190	1117190
	-	inside	IScluster/Tn	-	-	207827..217508	9681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T72547 WP_010967243.1 NZ_CP019486:215692-215985 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T72547 NZ_CP019486:215692-215985 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT72547 WP_010967242.1 NZ_CP019486:215387-215695 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT72547 NZ_CP019486:215387-215695 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: Q930F0

Antitoxin

• AlphaFold DB: Q930F1