72546

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3421560..3422221 Replicon chromosome
Accession NZ_CP019485
Organism Sinorhizobium meliloti strain B401

Toxin (Protein)


Gene name vapC Uniprot ID Q92KY5
Locus tag BWO76_RS30480 Protein ID WP_010970565.1
Coordinates 3421560..3421964 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q92KY4
Locus tag BWO76_RS30485 Protein ID WP_010970566.1
Coordinates 3421961..3422221 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWO76_RS30465 3417103..3418215 + 1113 WP_003535893.1 3-isopropylmalate dehydrogenase -
BWO76_RS30470 3418344..3419051 + 708 WP_010970563.1 phosphatase PAP2 family protein -
BWO76_RS30475 3419152..3421041 - 1890 WP_010970564.1 MHS family MFS transporter -
BWO76_RS30480 3421560..3421964 - 405 WP_010970565.1 type II toxin-antitoxin system VapC family toxin Toxin
BWO76_RS30485 3421961..3422221 - 261 WP_010970566.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
BWO76_RS30490 3422350..3422520 + 171 WP_010970567.1 hypothetical protein -
BWO76_RS30495 3422634..3423668 + 1035 WP_003531790.1 aspartate-semialdehyde dehydrogenase -
BWO76_RS30500 3423841..3424662 + 822 WP_010970568.1 lytic transglycosylase family protein -
BWO76_RS30510 3425009..3425692 - 684 WP_010970569.1 carbonic anhydrase -
BWO76_RS30515 3425866..3426747 - 882 WP_010970570.1 pyridoxal kinase PdxY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(8-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14507.71 Da        Isoelectric Point: 6.7257

>T72546 WP_010970565.1 NZ_CP019485:c3421964-3421560 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Download         Length: 405 bp

>T72546 NZ_CP019485:c3421964-3421560 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9903.16 Da        Isoelectric Point: 5.1253

>AT72546 WP_010970566.1 NZ_CP019485:c3422221-3421961 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Download         Length: 261 bp

>AT72546 NZ_CP019485:c3422221-3421961 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92KY5

Antitoxin

Source ID Structure
AlphaFold DB Q92KY4

References