Detailed information of TA system TA072546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3421560..3422221
• Replicon: chromosome
• Accession: NZ_CP019485
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92KY5
• Locus tag: BWO76_RS30480
• Protein ID: WP_010970565.1
• Coordinates: 3421560..3421964 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92KY4
• Locus tag: BWO76_RS30485
• Protein ID: WP_010970566.1
• Coordinates: 3421961..3422221 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS30465	3417103..3418215	+	1113	WP_003535893.1	3-isopropylmalate dehydrogenase	-
BWO76_RS30470	3418344..3419051	+	708	WP_010970563.1	phosphatase PAP2 family protein	-
BWO76_RS30475	3419152..3421041	-	1890	WP_010970564.1	MHS family MFS transporter	-
BWO76_RS30480	3421560..3421964	-	405	WP_010970565.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWO76_RS30485	3421961..3422221	-	261	WP_010970566.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BWO76_RS30490	3422350..3422520	+	171	WP_010970567.1	hypothetical protein	-
BWO76_RS30495	3422634..3423668	+	1035	WP_003531790.1	aspartate-semialdehyde dehydrogenase	-
BWO76_RS30500	3423841..3424662	+	822	WP_010970568.1	lytic transglycosylase family protein	-
BWO76_RS30510	3425009..3425692	-	684	WP_010970569.1	carbonic anhydrase	-
BWO76_RS30515	3425866..3426747	-	882	WP_010970570.1	pyridoxal kinase PdxY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (8-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14507.71 Da
• Isoelectric Point: 6.7257

>T72546 WP_010970565.1 NZ_CP019485:c3421964-3421560 [Sinorhizobium meliloti]
VISHILDTNAVIALIGRKSDALVTRVLHSPQGIIGLPSVVAYELYFGAQKSAKAQHNLETLRLLMADFPILDFDRNDAFV
AGEIRAALAAKGTPIGPYDVLIAGQAKARGLTLVTNNVGEFNRVENLRVEDWSL

Nucleotide

• Length: 405 bp

>T72546 NZ_CP019485:c3421964-3421560 [Sinorhizobium meliloti]
GTGATCTCCCATATTCTCGACACCAACGCCGTCATCGCTTTGATCGGGCGCAAATCGGATGCATTGGTCACCCGTGTACT
CCATAGCCCTCAGGGCATTATCGGGCTCCCGTCGGTCGTCGCGTATGAACTCTATTTTGGAGCGCAGAAAAGCGCGAAGG
CCCAACACAATCTCGAAACCCTGCGTTTGTTGATGGCGGATTTTCCGATCCTCGACTTCGACCGGAACGATGCCTTTGTC
GCAGGCGAGATCCGGGCCGCCCTTGCCGCGAAGGGTACGCCCATCGGACCTTATGACGTGCTGATTGCGGGACAGGCCAA
AGCCCGTGGATTGACGCTCGTCACCAACAATGTCGGCGAGTTCAATAGGGTTGAGAACCTGCGCGTGGAGGATTGGTCGC
TCTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9903.16 Da
• Isoelectric Point: 5.1253

>AT72546 WP_010970566.1 NZ_CP019485:c3422221-3421961 [Sinorhizobium meliloti]
MPHLARVFQSGNSQAVRLPKEFRFNVDRVEITQEGDALILRPHVEQGEQWSSLKAALARGMSEDFMMCGREQPEQQDRSE
LDAVFR

Nucleotide

• Length: 261 bp

>AT72546 NZ_CP019485:c3422221-3421961 [Sinorhizobium meliloti]
ATGCCCCATCTTGCACGGGTGTTCCAGTCCGGAAATTCGCAGGCCGTCCGCCTGCCTAAGGAATTCCGCTTCAACGTCGA
TCGCGTCGAAATCACGCAGGAAGGCGACGCGCTCATTCTCCGCCCGCACGTCGAGCAAGGCGAGCAGTGGTCATCGCTGA
AGGCGGCACTTGCCCGTGGCATGAGTGAAGACTTCATGATGTGCGGGCGCGAGCAGCCGGAACAGCAGGATCGGTCGGAA
CTCGACGCGGTATTTCGGTGA

Structures

Toxin

• AlphaFold DB: Q92KY5

Antitoxin

• AlphaFold DB: Q92KY4