Detailed information of TA system TA072545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2779912..2780543
• Replicon: chromosome
• Accession: NZ_CP019485
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWO76_RS27525
• Protein ID: WP_010970128.1
• Coordinates: 2779912..2780310 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q92MD9
• Locus tag: BWO76_RS27530
• Protein ID: WP_010970129.1
• Coordinates: 2780310..2780543 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS27490	2774995..2775297	-	303	WP_010970122.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BWO76_RS27495	2775294..2775569	-	276	WP_010970123.1	type II toxin-antitoxin system ParD family antitoxin	-
BWO76_RS27500	2775854..2776003	+	150	WP_010970124.1	hypothetical protein	-
BWO76_RS27505	2776000..2776935	+	936	WP_010970125.1	site-specific tyrosine recombinase XerD	-
BWO76_RS27510	2776999..2777952	+	954	WP_003527479.1	acetyl-CoA carboxylase carboxyltransferase subunit alpha	-
BWO76_RS27515	2778197..2779639	+	1443	WP_010970126.1	murein L,D-transpeptidase	-
BWO76_RS27520	2779639..2779878	+	240	WP_010970127.1	sulfurtransferase TusA family protein	-
BWO76_RS27525	2779912..2780310	-	399	WP_010970128.1	type II toxin-antitoxin system toxin VapC	Toxin
BWO76_RS27530	2780310..2780543	-	234	WP_010970129.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BWO76_RS27535	2780652..2781797	-	1146	WP_010970130.1	GTP-binding protein	-
BWO76_RS27540	2781811..2782893	-	1083	WP_010970131.1	D-alanyl-D-alanine carboxypeptidase	-
BWO76_RS27545	2783145..2784308	+	1164	WP_010970132.1	amidohydrolase	-
BWO76_RS27550	2784331..2784795	+	465	WP_010970133.1	Lrp/AsnC ligand binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14859.16 Da
• Isoelectric Point: 6.6329

>T72545 WP_010970128.1 NZ_CP019485:c2780310-2779912 [Sinorhizobium meliloti]
MLTYMLDTNICIYVMKTYPPAVREKFNGLAEQLCISSITLGELHYGAEKSAWRVENLTAIEHFVARLEVLPFADKAAAHY
GQVRAELERTGTPCGPHDMQIGAHARSEGLIVVTNNIREFVRMPGVRVENWL

Nucleotide

• Length: 399 bp

>T72545 NZ_CP019485:c2780310-2779912 [Sinorhizobium meliloti]
ATGCTCACCTATATGCTCGATACCAATATCTGCATCTATGTGATGAAGACCTATCCGCCGGCCGTGCGCGAAAAGTTCAA
CGGACTGGCGGAGCAGCTTTGCATATCGAGCATCACGCTGGGAGAATTACACTATGGCGCCGAAAAATCGGCCTGGCGCG
TTGAGAACCTTACGGCTATCGAACATTTCGTGGCACGGCTGGAGGTGCTGCCGTTCGCAGACAAGGCAGCGGCCCACTAC
GGGCAGGTTCGGGCTGAACTGGAGCGGACGGGAACGCCATGCGGCCCTCATGACATGCAGATCGGCGCGCATGCGCGCAG
CGAAGGGCTGATTGTCGTGACCAACAATATACGCGAGTTCGTCCGCATGCCGGGTGTAAGGGTCGAGAACTGGCTGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8673.87 Da
• Isoelectric Point: 8.9167

>AT72545 WP_010970129.1 NZ_CP019485:c2780543-2780310 [Sinorhizobium meliloti]
MASSTVFISNRSQAVRLPKAVAFPEGVHQVDILKIGRSRVIVPQGKRWDDLFLSGPRVSEDFMSERDQPVAETRESF

Nucleotide

• Length: 234 bp

>AT72545 NZ_CP019485:c2780543-2780310 [Sinorhizobium meliloti]
TTGGCCAGTTCGACCGTATTCATCAGTAACCGTAGCCAGGCGGTGCGGCTGCCAAAGGCCGTCGCCTTTCCCGAGGGCGT
GCATCAGGTCGATATCCTCAAGATCGGCCGCAGCCGCGTGATCGTCCCCCAGGGCAAAAGATGGGACGATCTGTTCCTCA
GCGGGCCGCGCGTCAGCGAGGATTTCATGAGCGAGCGTGATCAGCCGGTGGCGGAAACACGCGAATCCTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q92MD9