Detailed information of TA system TA072543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2569220..2569893
• Replicon: chromosome
• Accession: NZ_CP019485
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWO76_RS26460
• Protein ID: WP_010969926.1
• Coordinates: 2569489..2569893 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0GA10
• Locus tag: BWO76_RS26455
• Protein ID: WP_003536345.1
• Coordinates: 2569220..2569492 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS26435	2565211..2566851	-	1641	WP_003536336.1	ABC transporter substrate-binding protein	-
BWO76_RS26440	2566899..2567954	-	1056	WP_010969924.1	dipeptidase	-
BWO76_RS26445	2568134..2568475	-	342	WP_003536339.1	EamA family transporter	-
BWO76_RS26450	2568523..2569083	-	561	WP_010969925.1	TetR/AcrR family transcriptional regulator	-
BWO76_RS26455	2569220..2569492	+	273	WP_003536345.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BWO76_RS26460	2569489..2569893	+	405	WP_010969926.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWO76_RS26465	2570021..2570413	+	393	WP_003536349.1	globin	-
BWO76_RS26470	2570419..2570793	+	375	WP_010969927.1	DUF423 domain-containing protein	-
BWO76_RS26475	2570916..2571176	+	261	WP_010969928.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BWO76_RS26480	2571291..2571605	-	315	WP_003536354.1	antibiotic biosynthesis monooxygenase	-
BWO76_RS26485	2571618..2571884	-	267	WP_010969929.1	hypothetical protein	-
BWO76_RS26490	2571937..2572350	-	414	WP_003536357.1	DUF2325 domain-containing protein	-
BWO76_RS26495	2572474..2573400	-	927	WP_010969930.1	energy transducer TonB	-
BWO76_RS26500	2573441..2573875	-	435	WP_010969931.1	hypothetical protein	-
BWO76_RS26505	2574105..2574245	+	141	WP_010969932.1	hemin uptake protein HemP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-54)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14747.98 Da
• Isoelectric Point: 5.1974

>T72543 WP_010969926.1 NZ_CP019485:2569489-2569893 [Sinorhizobium meliloti]
MNGYLLDTNIISDVIHNPFGPAAQRIERIGPKEIYTSIVVASELRYGCAKKGSAKLLAKVESLLEIVPVLPLDIPADTRY
GSIRAELESLGQTIGSNDLLIAAHAYALDLTLVTDNIREFSRVRGLSLENWLER

Nucleotide

• Length: 405 bp

>T72543 NZ_CP019485:2569489-2569893 [Sinorhizobium meliloti]
TTGAACGGCTATCTTCTCGATACGAACATCATCAGCGACGTCATTCACAATCCGTTCGGACCGGCTGCGCAACGCATCGA
ACGGATCGGCCCGAAGGAGATTTACACCAGCATCGTCGTCGCGTCCGAGTTGCGGTATGGTTGCGCAAAAAAGGGATCGG
CGAAGTTGCTCGCCAAGGTCGAAAGCTTGCTGGAAATCGTCCCGGTGCTGCCATTGGATATTCCGGCCGACACCCGATAT
GGCAGCATTCGTGCGGAATTGGAGTCTCTGGGGCAGACCATCGGCTCCAACGATCTGTTGATTGCCGCGCATGCATATGC
GCTGGACCTGACGCTGGTGACGGACAATATCCGTGAGTTCAGCCGCGTTCGTGGCCTTAGTCTGGAAAACTGGCTTGAGC
GATAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10334.02 Da
• Isoelectric Point: 6.3950

>AT72543 WP_003536345.1 NZ_CP019485:2569220-2569492 [Sinorhizobium meliloti]
MPVPLPSSRPKEVKLFRNNRSQAVRIPAEFELPGDRVLIRREGTRLIIEPIARPADIVELLAEWKKEAPLGPEDRFPDVE
DIPARPEKIF

Nucleotide

• Length: 273 bp

>AT72543 NZ_CP019485:2569220-2569492 [Sinorhizobium meliloti]
ATGCCCGTCCCGTTGCCGTCATCGCGACCTAAAGAAGTAAAGCTGTTCCGCAATAACCGCAGCCAGGCAGTCCGTATTCC
TGCGGAGTTCGAGTTGCCTGGAGACCGTGTGTTGATCCGTCGCGAAGGAACGAGACTCATTATCGAACCGATAGCCAGGC
CAGCCGATATCGTGGAGCTTCTCGCGGAGTGGAAGAAGGAAGCTCCCCTCGGACCTGAAGACCGGTTCCCGGATGTTGAG
GATATACCCGCCCGGCCGGAGAAAATCTTTTGA

Structures

Antitoxin

• AlphaFold DB: H0GA10