Detailed information of TA system TA072542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2158529..2159183
• Replicon: chromosome
• Accession: NZ_CP019485
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H0G1A3
• Locus tag: BWO76_RS24475
• Protein ID: WP_003530010.1
• Coordinates: 2158529..2158717 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: Q92NX0
• Locus tag: BWO76_RS24480
• Protein ID: WP_010969659.1
• Coordinates: 2158722..2159183 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS24460	2155095..2156405	+	1311	WP_010969656.1	HlyD family type I secretion periplasmic adaptor subunit	-
BWO76_RS24465	2156430..2157401	-	972	WP_017265202.1	glycosyltransferase family 2 protein	-
BWO76_RS24470	2157582..2158352	-	771	WP_010969658.1	hypothetical protein	-
BWO76_RS24475	2158529..2158717	+	189	WP_003530010.1	type II toxin-antitoxin system HicA family toxin	Toxin
BWO76_RS24480	2158722..2159183	+	462	WP_010969659.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BWO76_RS24490	2159502..2160419	-	918	WP_003530005.1	PhzF family phenazine biosynthesis protein	-
BWO76_RS24495	2160596..2161345	-	750	WP_010969660.1	NUDIX hydrolase	-
BWO76_RS24500	2161348..2162454	-	1107	WP_003530001.1	endonuclease	-
BWO76_RS24505	2162605..2163048	-	444	WP_003529999.1	TIGR02594 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-124)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 6971.15 Da
• Isoelectric Point: 11.0889

>T72542 WP_003530010.1 NZ_CP019485:2158529-2158717 [Sinorhizobium meliloti]
MKSGDIIAALQKDGWYEVATKGSHVQFKHPKKHGRVTVPHPKRDLPIGTLRSIEKQSGLKLR

Nucleotide

• Length: 189 bp

>T72542 NZ_CP019485:2158529-2158717 [Sinorhizobium meliloti]
ATGAAGAGCGGCGACATTATTGCAGCATTGCAGAAGGACGGATGGTACGAGGTTGCGACCAAGGGCAGCCACGTTCAATT
CAAACATCCGAAAAAGCACGGCCGGGTTACCGTTCCTCACCCGAAGAGGGATCTACCAATCGGTACCCTCAGGAGCATTG
AAAAGCAGTCCGGTTTGAAATTGAGGTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16293.17 Da
• Isoelectric Point: 4.3990

>AT72542 WP_010969659.1 NZ_CP019485:2158722-2159183 [Sinorhizobium meliloti]
MRNYIGLIHKDAESDYGVSFPDFSGVVTAGADLDDARAMAEEALALHIEGLVEDGEAIPEPSSLEVVMSDAENRDCVAIL
VAVKTEAKRAIRVNVTLPEGVLKQIDAFAEAHGLTRSGFLARAATHEIERANDGHDAYAESRLSALGTSSKNV

Nucleotide

• Length: 462 bp

>AT72542 NZ_CP019485:2158722-2159183 [Sinorhizobium meliloti]
ATGCGCAACTATATCGGATTGATCCACAAGGACGCCGAGAGCGATTACGGCGTTTCCTTTCCTGATTTTTCCGGCGTCGT
GACCGCCGGTGCCGACCTCGACGACGCGCGCGCTATGGCAGAGGAAGCCTTGGCCTTGCATATTGAAGGGCTGGTAGAGG
ACGGCGAGGCCATCCCGGAGCCCTCCTCCCTTGAGGTTGTCATGTCCGACGCCGAGAACAGGGATTGCGTCGCTATTCTC
GTCGCGGTGAAAACCGAGGCAAAAAGAGCCATTCGAGTGAACGTTACGCTTCCCGAAGGTGTCCTGAAGCAGATAGATGC
CTTCGCCGAAGCCCACGGCCTTACGCGGTCTGGATTTCTTGCTCGTGCTGCAACGCACGAGATCGAACGGGCAAATGACG
GCCACGACGCCTATGCGGAATCCCGCTTGTCGGCTTTGGGCACTTCCAGCAAAAATGTGTAA

Structures

Toxin

• AlphaFold DB: H0G1A3

Antitoxin

• AlphaFold DB: Q92NX0