Detailed information of TA system TA072539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 842432..843072
• Replicon: chromosome
• Accession: NZ_CP019485
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWO76_RS17815
• Protein ID: WP_095919927.1
• Coordinates: 842432..842818 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: H0G6N0
• Locus tag: BWO76_RS17820
• Protein ID: WP_003533689.1
• Coordinates: 842818..843072 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS17790	837896..838375	+	480	WP_003537307.1	GNAT family N-acetyltransferase	-
BWO76_RS17795	838419..838868	-	450	WP_003537305.1	nucleoside deaminase	-
BWO76_RS17800	838943..840664	+	1722	WP_010968830.1	pseudouridine synthase	-
BWO76_RS17805	840671..841231	+	561	WP_010968831.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
BWO76_RS17810	841304..842203	+	900	WP_010968832.1	patatin-like phospholipase family protein	-
BWO76_RS17815	842432..842818	-	387	WP_095919927.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWO76_RS17820	842818..843072	-	255	WP_003533689.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BWO76_RS17825	843211..845043	-	1833	WP_010968833.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
BWO76_RS17830	845195..846541	+	1347	WP_010968834.1	TldD/PmbA family protein	-
BWO76_RS17835	846650..847468	+	819	WP_010968835.1	3'(2'),5'-bisphosphate nucleotidase CysQ	-
BWO76_RS17840	847529..847765	+	237	WP_003533684.1	DUF4170 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-83)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13846.73 Da
• Isoelectric Point: 4.7010

>T72539 WP_095919927.1 NZ_CP019485:c842818-842432 [Sinorhizobium meliloti]
MVIDTSAIAAIAFNEQEAGSFREKIADDPVRLISAATALEAAMVIETRLGEAAGAELDLWLYKANVEIVAVTAEHMDQAR
RAWRRFGKGRHPAGLNFGDCFSYALAFLTNEPLLFKGSGFSQTDIPAA

Nucleotide

• Length: 387 bp

>T72539 NZ_CP019485:c842818-842432 [Sinorhizobium meliloti]
ATGGTCATCGACACATCCGCCATTGCCGCGATTGCCTTCAATGAACAGGAGGCCGGAAGTTTCCGCGAAAAAATCGCGGA
CGACCCGGTTCGGCTCATCTCCGCGGCAACCGCTCTGGAAGCGGCCATGGTAATCGAAACCCGGTTAGGCGAAGCTGCCG
GAGCGGAACTTGATCTTTGGCTTTACAAGGCAAACGTGGAGATTGTTGCGGTAACCGCCGAGCATATGGACCAGGCCCGC
CGTGCCTGGCGCCGTTTCGGCAAAGGCCGTCATCCGGCCGGTTTGAATTTCGGTGATTGTTTTTCCTATGCCCTGGCCTT
TCTGACAAATGAACCGCTTCTCTTCAAGGGAAGCGGTTTCTCGCAAACCGATATCCCGGCGGCATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9472.63 Da
• Isoelectric Point: 4.8660

>AT72539 WP_003533689.1 NZ_CP019485:c843072-842818 [Sinorhizobium meliloti]
MALSIKDTETEQLARTLARRTGESITLATKRALEERLRRTDTAARRAALLEDLEAIQRRWNAMPVLDSRSSDDIIGYDED
GLPS

Nucleotide

• Length: 255 bp

>AT72539 NZ_CP019485:c843072-842818 [Sinorhizobium meliloti]
ATGGCGCTGAGCATCAAGGACACTGAAACCGAGCAGCTTGCCCGCACACTTGCCCGGCGGACAGGGGAATCCATCACGCT
TGCCACCAAGCGTGCCCTCGAAGAACGATTGCGCCGAACCGACACCGCTGCCCGGCGCGCGGCTCTTCTGGAGGATCTGG
AAGCCATTCAACGCCGCTGGAATGCCATGCCCGTCCTCGACAGTCGCAGCTCCGATGACATTATCGGTTATGACGAGGAT
GGACTCCCCTCATAA

Structures

Antitoxin

• AlphaFold DB: H0G6N0