Detailed information of TA system TA072535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1056909..1057489
• Replicon: plasmid pSymA
• Accession: NZ_CP019483
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q92XP1
• Locus tag: BWO76_RS05800
• Protein ID: WP_010968151.1
• Coordinates: 1057106..1057489 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BWO76_RS05795
• Protein ID: WP_088199999.1
• Coordinates: 1056909..1057109 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS05760	1052520..1053260	+	741	WP_040120638.1	SDR family oxidoreductase	-
BWO76_RS05765	1053790..1054086	-	297	WP_095919042.1	hypothetical protein	-
BWO76_RS05770	1054086..1055708	-	1623	WP_164829720.1	IS66 family transposase	-
BWO76_RS05775	1055772..1056065	-	294	WP_015241282.1	IS66 family insertion sequence element accessory protein TnpB	-
BWO76_RS05785	1056279..1056653	-	375	WP_012477218.1	IS66 family insertion sequence hypothetical protein	-
BWO76_RS05795	1056909..1057109	+	201	WP_088199999.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
BWO76_RS05800	1057106..1057489	+	384	WP_010968151.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWO76_RS05805	1057737..1059342	+	1606	Protein_1042	transposase	-
BWO76_RS05815	1059344..1059664	-	321	WP_015241552.1	hypothetical protein	-
BWO76_RS05825	1060047..1061201	+	1155	WP_095926528.1	site-specific integrase	-
BWO76_RS05835	1061655..1061924	+	270	WP_017271873.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1096825	1096825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14292.54 Da
• Isoelectric Point: 6.9172

>T72535 WP_010968151.1 NZ_CP019483:1057106-1057489 [Sinorhizobium meliloti]
VILADTSIWIDHFRHTDAELRRIIEDDRLLCHPAVIGELALGSLRERSSVIAFLMAQREALVATHQEVMMMIDRHAIFSM
GIGYTDAHLLASVLLDQRMALWTRDKRLQAAAEKAGASLHTPAHTRN

Nucleotide

• Length: 384 bp

>T72535 NZ_CP019483:1057106-1057489 [Sinorhizobium meliloti]
GTGATTCTTGCCGACACTTCAATCTGGATTGATCATTTCCGGCATACCGATGCCGAGCTGCGTAGGATTATTGAGGACGA
TCGTCTCCTCTGCCATCCGGCCGTGATCGGAGAGCTTGCACTTGGCAGCCTTCGGGAACGCAGTAGCGTGATAGCATTCC
TGATGGCTCAGCGCGAAGCACTCGTCGCAACGCACCAGGAGGTCATGATGATGATCGATCGCCACGCCATTTTTAGCATG
GGGATTGGCTACACGGATGCCCATTTGCTGGCCTCGGTTCTCCTCGATCAGCGAATGGCTTTGTGGACGAGAGACAAGCG
TTTGCAGGCAGCGGCCGAAAAGGCGGGGGCGTCGCTGCACACCCCCGCCCATACGCGGAACTGA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7080.22 Da
• Isoelectric Point: 11.1107

>AT72535 WP_088199999.1 NZ_CP019483:1056909-1057109 [Sinorhizobium meliloti]
MRSTINLDDTLMERAKALTGTKETAALVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT72535 NZ_CP019483:1056909-1057109 [Sinorhizobium meliloti]
ATGCGATCGACTATCAACCTCGACGACACTCTCATGGAAAGGGCTAAGGCCCTAACCGGCACAAAAGAAACCGCCGCTCT
GGTCCGCCAGGCATTGGAGACGCTTGTTCGTGTGGAGTCAGGAAAACGCCTCATCGCGCTCGGGGGAACTATGCCGGATG
CGGAAGCAGCTCCACGCCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• AlphaFold DB: Q92XP1