Detailed information of TA system TA072534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 982706..983313
• Replicon: plasmid pSymA
• Accession: NZ_CP019483
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BWO76_RS05375
• Protein ID: WP_017264738.1
• Coordinates: 982706..982960 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: BWO76_RS05380
• Protein ID: WP_040120685.1
• Coordinates: 982957..983313 (+)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS05355	978107..978811	-	705	WP_014527995.1	haloacid dehalogenase type II	-
BWO76_RS05360	978902..979774	+	873	WP_010968043.1	LysR family transcriptional regulator	-
BWO76_RS30730	979825..979980	-	156	WP_153320124.1	hypothetical protein	-
BWO76_RS31085	980066..980224	-	159	WP_017276730.1	hypothetical protein	-
BWO76_RS05365	980348..981388	-	1041	WP_040120687.1	NAD(P)H-binding protein	-
BWO76_RS05370	981477..982364	+	888	WP_040120686.1	LysR family transcriptional regulator	-
BWO76_RS05375	982706..982960	+	255	WP_017264738.1	type II toxin-antitoxin system HicA family toxin	Toxin
BWO76_RS05380	982957..983313	+	357	WP_040120685.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BWO76_RS05385	983685..984341	-	657	WP_010968046.1	SGNH/GDSL hydrolase family protein	-
BWO76_RS05390	984371..985321	-	951	WP_010968047.1	ABC transporter permease	-
BWO76_RS05395	985324..986295	-	972	WP_040120683.1	ABC transporter permease	-
BWO76_RS05400	986312..987805	-	1494	WP_010968049.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1096825	1096825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9294.62 Da
• Isoelectric Point: 9.8124

>T72534 WP_017264738.1 NZ_CP019483:982706-982960 [Sinorhizobium meliloti]
MNSKQRKTLAAVFKDPASGTIEWAAIENLLVAAGADVIEGNGSRVKFEKDGIIASFHRPHPDKEAKRYQVRDAREFLMQI
GVTP

Nucleotide

• Length: 255 bp

>T72534 NZ_CP019483:982706-982960 [Sinorhizobium meliloti]
ATGAACAGCAAGCAGAGAAAGACCCTCGCGGCCGTATTCAAGGACCCGGCCTCGGGAACGATTGAATGGGCCGCTATAGA
GAATCTGCTTGTGGCTGCCGGAGCGGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGCATCATTG
CGAGCTTTCATCGACCTCATCCGGACAAGGAAGCAAAGCGTTATCAGGTGCGCGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13178.82 Da
• Isoelectric Point: 5.2288

>AT72534 WP_040120685.1 NZ_CP019483:982957-983313 [Sinorhizobium meliloti]
MNTMTYNGYHARIEFDAEDEVFFGKIAGISDVIGFHGDSVAELKKAFHEAVDDYLETCRKIGKEPQRPYSGKMMFRVAPE
VHRRAALAAELSGKSLNQWAEEVLEEAAGHFAEARLSA

Nucleotide

• Length: 357 bp

>AT72534 NZ_CP019483:982957-983313 [Sinorhizobium meliloti]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGAAGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATGTAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCGGTCGATGACT
ACCTTGAGACCTGCAGGAAAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTCAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGGCCATTTTGCCGAGGCGCGCCTGTCGGCGTAG