Detailed information of TA system TA072533

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 495204..495889
• Replicon: plasmid pSymA
• Accession: NZ_CP019483
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BWO76_RS02760
• Protein ID: WP_095918854.1
• Coordinates: 495204..495623 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: BWO76_RS02765
• Protein ID: WP_088196198.1
• Coordinates: 495620..495889 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS02740	490465..492102	+	1638	WP_014531600.1	arylsulfatase	-
BWO76_RS02745	492180..492599	+	420	Protein_485	hypothetical protein	-
BWO76_RS02750	492741..493535	-	795	Protein_486	IS256-like element ISRm3 family transposase	-
BWO76_RS30695	493529..493669	-	141	Protein_487	helix-turn-helix domain-containing protein	-
BWO76_RS02755	493857..494858	-	1002	WP_095926489.1	IS30 family transposase	-
BWO76_RS02760	495204..495623	-	420	WP_095918854.1	type II toxin-antitoxin system VapC family toxin	Toxin
BWO76_RS02765	495620..495889	-	270	WP_088196198.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BWO76_RS02775	496244..497117	-	874	Protein_491	LysR family transcriptional regulator	-
BWO76_RS02795	498103..498416	+	314	Protein_492	hypothetical protein	-
BWO76_RS02800	498524..498904	-	381	WP_095918856.1	transcriptional regulator	-
BWO76_RS02805	499057..500253	-	1197	WP_010969783.1	IS256-like element ISRm5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1096825	1096825
	-	inside	IScluster/Tn	-	-	492741..504193	11452

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (18-55)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14876.18 Da
• Isoelectric Point: 5.1858

>T72533 WP_095918854.1 NZ_CP019483:c495623-495204 [Sinorhizobium meliloti]
VSRLYMLDTNIVSELARNPQGAVTKRIAEIGPDAICVSIITAAELRYRCAKKGSSKLLAQIETILGSMQVLALDVPADAE
YGGIRAELEAAGKPIGPNDLFIAAHACMLGAVLVTANSSEFTRVRDLKVENWLDFTSQG

Nucleotide

• Length: 420 bp

>T72533 NZ_CP019483:c495623-495204 [Sinorhizobium meliloti]
GTGAGCAGGCTCTACATGCTGGACACCAATATCGTTTCCGAGCTTGCGCGAAACCCACAGGGGGCTGTCACTAAACGCAT
TGCCGAGATAGGACCGGATGCAATCTGCGTCAGCATCATTACGGCGGCAGAGTTACGCTATAGATGCGCCAAGAAGGGAT
CATCGAAACTGCTGGCGCAGATCGAGACCATTCTTGGAAGTATGCAAGTGCTTGCGCTCGATGTGCCTGCTGATGCCGAA
TACGGCGGCATCCGGGCTGAACTGGAGGCTGCTGGTAAGCCCATCGGCCCGAATGACCTGTTCATCGCCGCTCATGCCTG
TATGCTTGGAGCGGTGCTGGTAACGGCCAACAGTAGCGAGTTCACACGCGTTCGTGATCTCAAGGTCGAGAACTGGCTGG
ATTTCACTTCTCAGGGTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10202.68 Da
• Isoelectric Point: 4.5716

>AT72533 WP_088196198.1 NZ_CP019483:c495889-495620 [Sinorhizobium meliloti]
MPQLRHNEPTPPREAKLFRNNRSQAVRIPADFELPGDRVMIHRDGDRLIIEPVLRKNLLEVLAGLEPLGSEDEFPDVDET
LLPVKEIDL

Nucleotide

• Length: 270 bp

>AT72533 NZ_CP019483:c495889-495620 [Sinorhizobium meliloti]
ATGCCCCAACTCCGACACAACGAACCAACACCGCCAAGGGAAGCCAAGCTCTTCCGAAATAATAGAAGTCAGGCTGTACG
TATCCCCGCTGATTTTGAGCTTCCCGGCGATCGGGTGATGATCCATCGGGACGGAGATCGGCTGATAATAGAGCCGGTGC
TCCGCAAGAACTTGCTGGAAGTGCTGGCTGGTTTGGAGCCGCTCGGGTCTGAAGATGAGTTCCCTGACGTTGACGAAACC
CTGCTGCCGGTGAAGGAAATCGACCTGTGA