Detailed information of TA system TA072532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 230533..231131
• Replicon: plasmid pSymA
• Accession: NZ_CP019483
• Organism: Sinorhizobium meliloti strain B401

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q930F0
• Locus tag: BWO76_RS01270
• Protein ID: WP_010967243.1
• Coordinates: 230838..231131 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q930F1
• Locus tag: BWO76_RS01265
• Protein ID: WP_010967242.1
• Coordinates: 230533..230841 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWO76_RS01245	226734..228518	+	1785	WP_095918731.1	polysaccharide biosynthesis/export family protein	-
BWO76_RS01255	229558..229824	+	267	WP_010967197.1	EAL domain-containing protein	-
BWO76_RS01260	229976..230188	-	213	WP_013845891.1	hypothetical protein	-
BWO76_RS01265	230533..230841	+	309	WP_010967242.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BWO76_RS01270	230838..231131	+	294	WP_010967243.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
BWO76_RS01275	231364..232563	-	1200	WP_164828840.1	NAD-dependent formate dehydrogenase	-
BWO76_RS01280	232978..233565	+	588	Protein_236	RepB plasmid partition	-
BWO76_RS01285	233943..234719	-	777	WP_014528764.1	histidinol-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	htpB	1..1096825	1096825

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11278.93 Da
• Isoelectric Point: 6.4734

>T72532 WP_010967243.1 NZ_CP019483:230838-231131 [Sinorhizobium meliloti]
MRLVWARYALDDRDTIFSYIERENPRAAVHVDEEIVSAVRRLLDFPESGRPGRIAGTRELVIPRTPYIAAYMVMEDRIRI
LRVLHGAQKWPSELDDG

Nucleotide

• Length: 294 bp

>T72532 NZ_CP019483:230838-231131 [Sinorhizobium meliloti]
ATGAGGCTCGTCTGGGCGCGATATGCGCTTGACGATCGCGACACCATCTTCAGTTACATCGAAAGGGAGAATCCGAGGGC
GGCGGTCCACGTCGACGAGGAGATCGTCAGCGCGGTACGTCGTCTGCTTGATTTTCCGGAAAGCGGCCGCCCTGGCCGGA
TTGCCGGAACTCGAGAGCTTGTTATCCCTCGGACCCCCTACATTGCAGCCTACATGGTAATGGAGGATAGAATTCGAATT
CTGCGTGTCCTGCACGGCGCCCAGAAGTGGCCCAGCGAGCTCGACGACGGGTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11210.58 Da
• Isoelectric Point: 5.6604

>AT72532 WP_010967242.1 NZ_CP019483:230533-230841 [Sinorhizobium meliloti]
MASNALVQTRIDAEVKERATAVLENMGLTVSDAVRILLTRTANEGALPLELFSHSEAHDAWFRAKVLRALEDTRPDVDDA
DADAHFRERRAAALRKAAAGDR

Nucleotide

• Length: 309 bp

>AT72532 NZ_CP019483:230533-230841 [Sinorhizobium meliloti]
ATGGCTTCAAATGCACTTGTTCAGACTCGAATCGATGCCGAGGTCAAAGAGCGCGCCACGGCGGTTTTAGAGAACATGGG
TCTGACGGTCTCGGACGCTGTTCGAATCCTGTTAACTCGGACGGCGAATGAGGGCGCCCTGCCGCTGGAACTCTTCAGCC
ACAGCGAGGCGCACGACGCTTGGTTCCGCGCCAAAGTGTTGCGGGCACTCGAGGATACCCGACCCGACGTCGACGATGCC
GACGCCGATGCGCACTTTCGAGAGCGCCGGGCGGCAGCTTTGCGCAAGGCTGCGGCGGGTGATCGATGA

Structures

Toxin

• AlphaFold DB: Q930F0

Antitoxin

• AlphaFold DB: Q930F1