Detailed information of TA system TA072506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 125070..125695
• Replicon: plasmid pFHI_NMBU_03_1
• Accession: NZ_CP019456
• Organism: Escherichia coli strain FHI_NMBU_03

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BXO92_RS24540
• Protein ID: WP_021572485.1
• Coordinates: 125297..125695 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: BXO92_RS24535
• Protein ID: WP_000450532.1
• Coordinates: 125070..125297 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO92_RS24535	125070..125297	+	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin
BXO92_RS24540	125297..125695	+	399	WP_021572485.1	tRNA(fMet)-specific endonuclease VapC	Toxin
BXO92_RS24545	125704..127857	-	2154	WP_104934212.1	type IV conjugative transfer system coupling protein TraD	-
BXO92_RS24550	128110..128841	-	732	WP_024244211.1	complement resistance protein TraT	-
BXO92_RS24555	128873..129370	-	498	WP_021572404.1	surface exclusion inner membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC	1..159821	159821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14858.13 Da
• Isoelectric Point: 7.8605

>T72506 WP_021572485.1 NZ_CP019456:125297-125695 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNDTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T72506 NZ_CP019456:125297-125695 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTTACGATAAAGAACAAACCCGCCAGTGTCAGGGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGCATCAGTTCGGTCACTCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGACGTTCTGGATTACGACGCTGCTGCTGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATGACACCCGGGAATTTGAACGTGTGGGCGGCCTGAGAATTGAAGACTGGAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT72506 WP_000450532.1 NZ_CP019456:125070-125297 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT72506 NZ_CP019456:125070-125297 [Escherichia coli]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGAATCATTACGCCTGCCGGAGAGACATGGGACGAATGGTTCGACGGAA
ACAGTGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCTGGTATGCAGGAAAGGGAGTCATTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5