Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 85238..85763 Replicon plasmid pFHI_NMBU_03_1
Accession NZ_CP019456
Organism Escherichia coli strain FHI_NMBU_03

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag BXO92_RS24310 Protein ID WP_001159871.1
Coordinates 85238..85543 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag BXO92_RS24315 Protein ID WP_000813639.1
Coordinates 85545..85763 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BXO92_RS24285 81128..81799 - 672 WP_024244225.1 response regulator transcription factor HprR -
BXO92_RS24290 81931..82344 + 414 WP_021541665.1 hydroxyisourate hydrolase -
BXO92_RS24295 82443..83435 + 993 WP_021541666.1 protein-methionine-sulfoxide reductase catalytic subunit MsrP -
BXO92_RS24300 83436..84071 + 636 WP_021541667.1 protein-methionine-sulfoxide reductase heme-binding subunit MsrQ -
BXO92_RS24305 84431..85237 - 807 WP_021541668.1 site-specific integrase -
BXO92_RS24310 85238..85543 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
BXO92_RS24315 85545..85763 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
BXO92_RS24325 86360..86617 + 258 WP_021564233.1 hypothetical protein -
BXO92_RS24330 86617..87207 + 591 WP_021564232.1 hypothetical protein -
BXO92_RS24335 87590..88768 - 1179 WP_021572468.1 hypothetical protein -
BXO92_RS24340 88816..89331 - 516 WP_021572469.1 type 1 fimbrial protein -
BXO92_RS24345 89398..90084 - 687 WP_021572470.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC 1..159821 159821


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T72503 WP_001159871.1 NZ_CP019456:c85543-85238 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T72503 NZ_CP019456:c85543-85238 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT72503 WP_000813639.1 NZ_CP019456:c85763-85545 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT72503 NZ_CP019456:c85763-85545 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACTATGCAGAACGAAGCCCGGCGACTGCGCGCAGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References