Detailed information of TA system TA072502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 5228..5763
• Replicon: plasmid pFHI_NMBU_03_1
• Accession: NZ_CP019456
• Organism: Escherichia coli strain FHI_NMBU_03

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BXO92_RS23955
• Protein ID: WP_001270421.1
• Coordinates: 5476..5763 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: L4J0V6
• Locus tag: BXO92_RS23950
• Protein ID: WP_001132895.1
• Coordinates: 5228..5479 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO92_RS23915	768..959	-	192	WP_001027495.1	hypothetical protein	-
BXO92_RS25020	963..2205	+	1243	Protein_2	translesion error-prone DNA polymerase V subunit UmuC	-
BXO92_RS23930	2346..3308	-	963	WP_021572436.1	ParB/RepB/Spo0J family partition protein	-
BXO92_RS23935	3305..4510	-	1206	WP_000817642.1	AAA family ATPase	-
BXO92_RS25025	4881..5054	+	174	WP_021576053.1	hypothetical protein	-
BXO92_RS23950	5228..5479	+	252	WP_001132895.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BXO92_RS23955	5476..5763	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BXO92_RS23960	5765..6170	+	406	Protein_8	DDE-type integrase/transposase/recombinase	-
BXO92_RS23970	6814..7791	+	978	WP_001309253.1	RepB family plasmid replication initiator protein	-
BXO92_RS23975	8034..8774	-	741	WP_021576052.1	tyrosine-type recombinase/integrase	-
BXO92_RS23980	9994..10272	+	279	WP_001523382.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	papH / papC / papD / hlyB / iroB / iroC / iroD / iroE / iroN / faeJ / faeI / faeH / faeF / faeF / faeE / faeD / faeC	1..159821	159821

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T72502 WP_001270421.1 NZ_CP019456:5476-5763 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T72502 NZ_CP019456:5476-5763 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAAACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9178.47 Da
• Isoelectric Point: 4.2108

>AT72502 WP_001132895.1 NZ_CP019456:5228-5479 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT72502 NZ_CP019456:5228-5479 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACTTGGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: L4J0V6