Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2723226..2723776 Replicon chromosome
Accession NZ_CP019455
Organism Escherichia coli strain FHI_NMBU_03

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0K4H9R5
Locus tag BXO92_RS13645 Protein ID WP_024223381.1
Coordinates 2723462..2723776 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag BXO92_RS13640 Protein ID WP_000125566.1
Coordinates 2723226..2723459 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BXO92_RS13615 2718299..2718586 + 288 WP_000203741.1 ferredoxin-like protein FixX -
BXO92_RS13620 2718645..2719976 + 1332 WP_001183207.1 MFS transporter -
BXO92_RS13625 2720084..2720614 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
BXO92_RS13630 2720607..2722469 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
BXO92_RS13635 2722661..2723140 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
BXO92_RS13640 2723226..2723459 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
BXO92_RS13645 2723462..2723776 + 315 WP_024223381.1 CcdB family protein Toxin
BXO92_RS13650 2723773..2724621 - 849 WP_104934099.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
BXO92_RS13655 2724628..2725005 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
BXO92_RS13660 2725008..2725829 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
BXO92_RS13665 2725826..2726815 - 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
BXO92_RS13670 2726815..2728101 - 1287 WP_104934100.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T72489 WP_024223381.1 NZ_CP019455:2723462-2723776 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMVDGI

Download         Length: 315 bp

>T72489 NZ_CP019455:2723462-2723776 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGATAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGGTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT72489 WP_000125566.1 NZ_CP019455:2723226-2723459 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT72489 NZ_CP019455:2723226-2723459 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4H9R5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References