Detailed information of TA system TA072486

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 2526345..2527052
• Replicon: chromosome
• Accession: NZ_CP019455
• Organism: Escherichia coli strain FHI_NMBU_03

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A4T3Q6D2
• Locus tag: BXO92_RS12705
• Protein ID: WP_001683515.1
• Coordinates: 2526711..2527052 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3L5H4Q7
• Locus tag: BXO92_RS12700
• Protein ID: WP_029696721.1
• Coordinates: 2526345..2526680 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BXO92_RS12675	2522291..2522509	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
BXO92_RS12680	2522627..2523241	-	615	WP_000772910.1	inovirus Gp2 family protein	-
BXO92_RS12685	2523834..2524787	+	954	WP_024223205.1	hypothetical protein	-
BXO92_RS12690	2525024..2525842	+	819	WP_024223204.1	DUF945 domain-containing protein	-
BXO92_RS12695	2525872..2526345	+	474	WP_001552321.1	DNA repair protein RadC	-
BXO92_RS12700	2526345..2526680	+	336	WP_029696721.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
BXO92_RS12705	2526711..2527052	+	342	WP_001683515.1	TA system toxin CbtA family protein	Toxin
BXO92_RS12710	2527167..2528000	+	834	WP_024223202.1	DUF4942 domain-containing protein	-
BXO92_RS12715	2528077..2528325	+	249	WP_024187601.1	ribbon-helix-helix domain-containing protein	-
BXO92_RS12720	2528329..2528604	+	276	WP_001683517.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BXO92_RS12725	2528718..2529515	+	798	WP_024223201.1	helix-turn-helix transcriptional regulator	-
BXO92_RS12730	2529845..2531110	+	1266	WP_054486255.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	2508654..2559817	51163

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-110)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13034.05 Da
• Isoelectric Point: 9.5454

>T72486 WP_001683515.1 NZ_CP019455:2526711-2527052 [Escherichia coli]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCHNTTAR

Nucleotide

• Length: 342 bp

>T72486 NZ_CP019455:2526711-2527052 [Escherichia coli]
ATGAAAATTATACCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGAATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGACAGGCTACAGGTCTGCTGCGCCGCTG
CCATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12572.62 Da
• Isoelectric Point: 7.6388

>AT72486 WP_029696721.1 NZ_CP019455:2526345-2526680 [Escherichia coli]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPCQQR
TVTLYAKGLTCEADTLGCCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT72486 NZ_CP019455:2526345-2526680 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTTACACCACGCTTCACTGCTCGACTGGT
GCAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGTGAAGCCGATACGCTGGGTTGCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: A0A4T3Q6D2

Antitoxin

• AlphaFold DB: A0A3L5H4Q7