Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 932519..933173 Replicon chromosome
Accession NZ_CP019455
Organism Escherichia coli strain FHI_NMBU_03

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag BXO92_RS04590 Protein ID WP_000244781.1
Coordinates 932519..932926 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BXO92_RS04595 Protein ID WP_000354046.1
Coordinates 932907..933173 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BXO92_RS04570 928476..930209 - 1734 WP_024235067.1 single-stranded-DNA-specific exonuclease RecJ -
BXO92_RS04575 930215..930925 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BXO92_RS04580 930950..931846 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BXO92_RS04585 931958..932479 + 522 WP_001055874.1 flavodoxin FldB -
BXO92_RS04590 932519..932926 - 408 WP_000244781.1 protein YgfX Toxin
BXO92_RS04595 932907..933173 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BXO92_RS04600 933416..934396 + 981 WP_024223065.1 tRNA-modifying protein YgfZ -
BXO92_RS04605 934473..935132 - 660 WP_000250274.1 hemolysin III family protein -
BXO92_RS04610 935296..935607 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
BXO92_RS04615 935652..937085 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T72477 WP_000244781.1 NZ_CP019455:c932926-932519 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T72477 NZ_CP019455:c932926-932519 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT72477 WP_000354046.1 NZ_CP019455:c933173-932907 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT72477 NZ_CP019455:c933173-932907 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References