Detailed information of TA system TA072473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4655..5691
• Replicon: plasmid p888-76-1
• Accession: NZ_CP019446
• Organism: Kosakonia cowanii JCM 10956 = DSM 18146 strain 888-76

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: BWI95_RS22030
• Protein ID: WP_054804302.1
• Coordinates: 5116..5691 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: BWI95_RS22025
• Protein ID: WP_054804301.1
• Coordinates: 4655..5116 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI95_RS22000	173..232	-	60	Protein_0	putative Ig domain-containing protein	-
BWI95_RS22005	317..1081	-	765	WP_076770354.1	putative Ig domain-containing protein	-
BWI95_RS22010	1102..1923	-	822	WP_076770355.1	cadherin-like beta sandwich domain-containing protein	-
BWI95_RS22015	2279..2476	+	198	Protein_3	VapC toxin family PIN domain ribonuclease	-
BWI95_RS22020	3126..3905	+	780	WP_076770356.1	conjugal transfer protein TraX	-
BWI95_RS22025	4655..5116	+	462	WP_054804301.1	helix-turn-helix domain-containing protein	Antitoxin
BWI95_RS22030	5116..5691	+	576	WP_054804302.1	PIN domain-containing protein	Toxin
BWI95_RS22035	6004..6459	+	456	WP_076770357.1	hypothetical protein	-
BWI95_RS22040	6453..6746	+	294	WP_076770358.1	DUF1493 family protein	-
BWI95_RS23465	7040..7369	+	330	WP_076770359.1	hypothetical protein	-
BWI95_RS22050	8189..9097	-	909	WP_076770360.1	replication initiator protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..113443	113443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-114)

Antitoxin

• SinI-like, DNA-binding domain (84-129)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21392.68 Da
• Isoelectric Point: 4.6944

>T72473 WP_054804302.1 NZ_CP019446:5116-5691 [Kosakonia cowanii JCM 10956 = DSM 18146]
MNHTPYPVLLDACVLYPSFLRDLLIRLGLTGLYQPKWSASIENEWQRNLLANRTDLIEDQIKRTATLMNKAFPDAMVTGF
EPLINSIDLPDVDDRHVAAAAVRSNAEIIVTLNLRDFPAPALDAFGIEALHPDDFVMDLFDLNSALVLSAVTTQRSSLRK
PPVSVDDYLEALLKQGMAQTVKELSKYRILI

Nucleotide

• Length: 576 bp

>T72473 NZ_CP019446:5116-5691 [Kosakonia cowanii JCM 10956 = DSM 18146]
ATGAACCACACGCCTTATCCCGTACTTTTAGATGCTTGCGTACTGTACCCGTCATTTCTACGGGATTTACTGATTCGATT
GGGGCTGACCGGGTTGTACCAACCAAAATGGTCAGCCAGTATCGAAAATGAGTGGCAACGTAACCTTTTAGCAAACAGAA
CGGATCTAATTGAAGATCAAATTAAACGCACTGCCACGTTAATGAATAAAGCCTTTCCTGATGCGATGGTCACCGGCTTT
GAGCCTCTGATTAATAGCATTGATTTACCTGATGTAGATGATCGGCATGTTGCCGCAGCAGCTGTCCGTAGCAATGCTGA
AATCATTGTGACGTTGAATCTTAGAGATTTTCCGGCACCTGCTCTTGATGCCTTTGGTATCGAAGCGTTGCATCCCGACG
ATTTTGTTATGGATTTATTCGATTTAAATAGTGCACTTGTGCTTTCGGCCGTAACTACGCAGCGCAGTAGTTTACGGAAA
CCGCCCGTGTCGGTTGATGATTATCTAGAAGCCCTGCTAAAGCAAGGTATGGCGCAAACCGTAAAAGAATTAAGCAAATA
CCGCATACTGATTTAG

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16907.43 Da
• Isoelectric Point: 6.3347

>AT72473 WP_054804301.1 NZ_CP019446:4655-5116 [Kosakonia cowanii JCM 10956 = DSM 18146]
MTNSILDSLSLPAKGEIEAAVRGQRELAVYLSTKMETQKISIQDADNVTHQIELPTSSLTLLMSILGELALGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEDGKLPFHKTGRHRRVLFADLMKYKGQRDSDSNKAMQELADLSQELGLY

Nucleotide

• Length: 462 bp

>AT72473 NZ_CP019446:4655-5116 [Kosakonia cowanii JCM 10956 = DSM 18146]
ATGACAAACTCAATTCTTGATAGCCTTAGCCTTCCGGCTAAAGGGGAGATTGAGGCAGCTGTACGTGGACAAAGGGAGCT
TGCCGTCTACCTCTCCACAAAAATGGAAACACAGAAAATTTCGATTCAGGATGCAGATAACGTAACTCATCAAATCGAAT
TACCGACGTCTTCACTGACATTGCTTATGTCGATTTTGGGTGAACTAGCACTTGGTAACGCGGTGCAAGTCGTTCCTGTG
CATGCTGAATTGACCACGCAGGAAGCCGCCAATATTCTAAATGTTTCACGCCCTCATATGGTGAAACTGCTCGAAGATGG
GAAGCTTCCTTTTCATAAAACAGGTCGCCATCGCCGCGTGCTTTTCGCCGATTTGATGAAGTACAAAGGTCAGCGAGATA
GCGATAGCAATAAAGCTATGCAGGAGTTGGCAGATCTAAGCCAAGAGCTTGGACTTTACTAA