Detailed information of TA system TA072459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 1462741..1463260
• Replicon: chromosome
• Accession: NZ_CP019445
• Organism: Kosakonia cowanii JCM 10956 = DSM 18146 strain 888-76

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: BWI95_RS06735
• Protein ID: WP_054803376.1
• Coordinates: 1462979..1463260 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: -
• Locus tag: BWI95_RS06730
• Protein ID: WP_042717989.1
• Coordinates: 1462741..1462989 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWI95_RS06715	1458506..1459765	-	1260	WP_023480794.1	oligosaccharide MFS transporter	-
BWI95_RS06720	1459823..1461649	-	1827	WP_076769221.1	DUF2264 domain-containing protein	-
BWI95_RS06725	1461815..1462690	+	876	WP_054803377.1	AraC family transcriptional regulator	-
BWI95_RS06730	1462741..1462989	+	249	WP_042717989.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
BWI95_RS06735	1462979..1463260	+	282	WP_054803376.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BWI95_RS06740	1463614..1465506	-	1893	WP_042717986.1	DNA topoisomerase IV subunit B	-
BWI95_RS06745	1465546..1466121	-	576	WP_054803018.1	esterase YqiA	-
BWI95_RS06750	1466121..1466948	-	828	WP_076769222.1	3',5'-cyclic-AMP phosphodiesterase	-
BWI95_RS06755	1466973..1467395	-	423	WP_023480711.1	DUF1249 family protein	-
BWI95_RS06760	1467392..1468024	-	633	WP_023480789.1	ADP-ribose diphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10928.74 Da
• Isoelectric Point: 10.6500

>T72459 WP_054803376.1 NZ_CP019445:1462979-1463260 [Kosakonia cowanii JCM 10956 = DSM 18146]
MTYKLAFNVSALKEWKKLGSTVREQFKKKLQERLENPRVPAAQLHGRKDQYKIKLRGAGYRLVYKVEEEIITVTVIGVGK
RENDEIYTATRFR

Nucleotide

• Length: 282 bp

>T72459 NZ_CP019445:1462979-1463260 [Kosakonia cowanii JCM 10956 = DSM 18146]
ATGACCTATAAGCTGGCGTTCAACGTATCGGCATTAAAGGAGTGGAAGAAGCTGGGAAGCACCGTTCGCGAACAGTTCAA
AAAGAAGCTTCAGGAGCGGCTCGAAAACCCGCGCGTCCCGGCAGCACAGCTTCATGGGCGCAAAGATCAGTACAAAATTA
AGCTACGCGGTGCGGGATACCGGCTGGTCTATAAAGTCGAAGAGGAGATCATCACCGTGACGGTGATCGGTGTGGGCAAA
CGCGAGAACGATGAGATCTACACAGCCACACGCTTCAGATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9097.37 Da
• Isoelectric Point: 3.9490

>AT72459 WP_042717989.1 NZ_CP019445:1462741-1462989 [Kosakonia cowanii JCM 10956 = DSM 18146]
MPFQILTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYARLMEMLEDEELGRIADERADEPTIEVKLD
DL

Nucleotide

• Length: 249 bp

>AT72459 NZ_CP019445:1462741-1462989 [Kosakonia cowanii JCM 10956 = DSM 18146]
ATGCCATTCCAGATTCTGACGACCACCGCAGCCAGCATCACCGAACTGAAACGTGACCCGATGGGAACATTTAATGCCGG
TGAAGGCGCGCCGGTTGCCATTCTTAATCGCAATGAACCCGCGTTCTACTGCGTCCCGCCCGCGCTCTATGCACGCCTGA
TGGAGATGCTCGAAGACGAAGAGCTGGGGCGCATTGCGGACGAACGTGCTGATGAACCCACCATCGAGGTCAAGCTTGAT
GACCTATAA