Detailed information of TA system TA072452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 34612..35224
• Replicon: plasmid unnamed2
• Accession: NZ_CP019439
• Organism: Thioclava nitratireducens strain 25B10_4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BMG03_RS20130
• Protein ID: WP_075777233.1
• Coordinates: 34612..35007 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1M7KKN2
• Locus tag: BMG03_RS20135
• Protein ID: WP_009815510.1
• Coordinates: 34997..35224 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMG03_RS20095	29618..30136	-	519	Protein_34	MFS transporter	-
BMG03_RS20100	30145..30366	+	222	WP_077701449.1	rhodanese-like domain-containing protein	-
BMG03_RS20105	30405..30617	-	213	WP_075777237.1	hypothetical protein	-
BMG03_RS20110	30614..31332	-	719	Protein_37	hypothetical protein	-
BMG03_RS20115	31414..32319	+	906	WP_075777236.1	MurR/RpiR family transcriptional regulator	-
BMG03_RS20120	32438..33505	-	1068	WP_075777235.1	hypothetical protein	-
BMG03_RS20125	33732..34628	+	897	WP_075777234.1	recombinase family protein	-
BMG03_RS20130	34612..35007	-	396	WP_075777233.1	PIN domain-containing protein	Toxin
BMG03_RS20135	34997..35224	-	228	WP_009815510.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BMG03_RS20140	35532..36719	+	1188	WP_075777232.1	plasmid partitioning protein RepA	-
BMG03_RS20145	36716..37732	+	1017	WP_075777231.1	plasmid partitioning protein RepB	-
BMG03_RS20150	37910..39145	+	1236	WP_075777230.1	replication initiation protein RepC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..114001	114001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-113)

Antitoxin

• Antitoxin PemI-like (2-46)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14365.47 Da
• Isoelectric Point: 4.3697

>T72452 WP_075777233.1 NZ_CP019439:c35007-34612 [Thioclava nitratireducens]
MSAEFADTNVVLYLLDDGPKAERVEEILGQGPRISVQVLNEAMVNCRRKAGLSWEDTGAFLEGIKSLCPVEGLTVQTHQV
GRALAEKYQLSVYDAMIVSAALIAGCTTLWTEDMHDGLLVEDRLRIVNPFA

Nucleotide

• Length: 396 bp

>T72452 NZ_CP019439:c35007-34612 [Thioclava nitratireducens]
ATGAGCGCTGAATTCGCAGACACGAATGTTGTGCTCTACCTGCTCGACGATGGTCCAAAGGCTGAGAGGGTGGAGGAAAT
TCTGGGGCAGGGACCCCGGATCAGTGTTCAGGTCCTGAACGAAGCGATGGTCAATTGCCGACGGAAAGCTGGGCTCAGTT
GGGAAGATACCGGAGCGTTTCTTGAGGGTATTAAGTCTTTATGCCCTGTCGAGGGCCTAACGGTTCAAACCCATCAAGTC
GGTCGCGCGTTGGCAGAGAAGTACCAGTTATCGGTCTATGACGCGATGATCGTGTCTGCCGCGCTGATCGCTGGGTGCAC
GACGTTGTGGACTGAGGACATGCACGACGGATTGCTGGTCGAGGATCGGCTGCGCATCGTCAATCCATTTGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8500.78 Da
• Isoelectric Point: 10.8838

>AT72452 WP_009815510.1 NZ_CP019439:c35224-34997 [Thioclava nitratireducens]
MQVAKWGNSLAVRLPADLVRELGLKEGDQVDLVKDDGTILVRRQPRADEVLQGLRRFRGKLPKDARLSRDAAHER

Nucleotide

• Length: 228 bp

>AT72452 NZ_CP019439:c35224-34997 [Thioclava nitratireducens]
ATGCAAGTTGCCAAATGGGGAAACTCCCTTGCCGTCCGGCTACCCGCGGATCTTGTGAGGGAACTCGGCTTAAAGGAAGG
CGATCAGGTCGACCTGGTAAAGGACGATGGAACCATTCTTGTGAGACGCCAACCTCGCGCCGATGAAGTTCTACAAGGGC
TAAGGCGGTTTCGCGGAAAACTGCCCAAGGACGCGCGTCTAAGCCGCGACGCCGCGCATGAGCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1M7KKN2