Detailed information of TA system TA072448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2505508..2506112
• Replicon: chromosome
• Accession: NZ_CP019432
• Organism: Pseudomonas sp. S49

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7Z2M765
• Locus tag: PspS49_RS11110
• Protein ID: WP_038369612.1
• Coordinates: 2505819..2506112 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PspS49_RS11105
• Protein ID: WP_122600417.1
• Coordinates: 2505508..2505816 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspS49_RS11085	2500854..2501957	+	1104	WP_160039249.1	SCO family protein	-
PspS49_RS11090	2502052..2503542	+	1491	WP_160039251.1	two-component sensor histidine kinase	-
PspS49_RS11095	2503511..2504953	+	1443	WP_160039253.1	sigma-54 dependent transcriptional regulator	-
PspS49_RS11100	2505104..2505451	-	348	WP_160039255.1	hypothetical protein	-
PspS49_RS11105	2505508..2505816	-	309	WP_122600417.1	putative addiction module antidote protein	Antitoxin
PspS49_RS11110	2505819..2506112	-	294	WP_038369612.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PspS49_RS11115	2506318..2507115	+	798	WP_160039257.1	class I SAM-dependent methyltransferase	-
PspS49_RS11120	2507252..2507944	-	693	WP_160039259.1	N-acetyltransferase	-
PspS49_RS11125	2507941..2509131	-	1191	WP_160039261.1	GNAT family N-acetyltransferase	-
PspS49_RS11130	2509136..2509825	-	690	WP_160039263.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (24-89)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10656.23 Da
• Isoelectric Point: 10.9839

>T72448 WP_038369612.1 NZ_CP019432:c2506112-2505819 [Pseudomonas sp. S49]
MNYLIRQTAVFQAWHQSVRDLRAKVAIARRIDRASTGNLGDVKSVGDGVSEMRVDVGAGYRVYFTMRNGAVIVLLAGGDK
SSQSADIRRAQKMAKEV

Nucleotide

• Length: 294 bp

>T72448 NZ_CP019432:c2506112-2505819 [Pseudomonas sp. S49]
ATGAATTATCTTATTCGACAAACAGCCGTCTTCCAGGCTTGGCACCAATCGGTTCGAGACCTGCGGGCCAAGGTGGCCAT
CGCCCGCCGCATTGATCGGGCCTCAACGGGAAATCTCGGCGATGTTAAATCGGTGGGTGATGGCGTATCTGAAATGCGCG
TAGATGTGGGTGCAGGCTATCGGGTGTATTTCACGATGCGTAACGGTGCAGTCATCGTTCTATTGGCTGGAGGCGACAAG
TCTTCACAAAGCGCAGACATCCGACGCGCACAAAAAATGGCTAAGGAGGTTTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11109.79 Da
• Isoelectric Point: 5.5740

>AT72448 WP_122600417.1 NZ_CP019432:c2505816-2505508 [Pseudomonas sp. S49]
MSQTLTIFDVAALLDSDEAISEYLSQVLADGDNEEFLRAIGYVLKARGMTQIAKTSGMGRESLYKAFAPGAKPRFETVLK
VMHALGIDLYAQPGHVINHPVV

Nucleotide

• Length: 309 bp

>AT72448 NZ_CP019432:c2505816-2505508 [Pseudomonas sp. S49]
ATGAGCCAGACGTTAACGATTTTCGACGTGGCAGCATTGCTGGACAGTGACGAAGCCATCAGCGAATACCTGTCCCAGGT
TCTTGCAGACGGGGATAACGAAGAGTTTCTTCGCGCAATCGGCTATGTGCTCAAGGCGCGCGGAATGACGCAGATAGCCA
AAACTTCTGGCATGGGTCGCGAAAGTCTGTACAAGGCGTTCGCGCCCGGCGCAAAGCCGCGCTTCGAAACGGTTTTGAAA
GTTATGCATGCCCTCGGCATCGATCTTTACGCACAGCCGGGGCATGTGATCAATCACCCGGTTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A7Z2M765