Detailed information of TA system TA072445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1605701..1606298
• Replicon: chromosome
• Accession: NZ_CP019432
• Organism: Pseudomonas sp. S49

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PspS49_RS07080
• Protein ID: WP_150773830.1
• Coordinates: 1606143..1606298 (-)
• Length: 52 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PspS49_RS07075
• Protein ID: WP_137218087.1
• Coordinates: 1605701..1606108 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspS49_RS07050	1601307..1602077	+	771	WP_150749329.1	trans-aconitate 2-methyltransferase	-
PspS49_RS07055	1602256..1602984	-	729	WP_150749328.1	class I SAM-dependent methyltransferase	-
PspS49_RS07060	1603549..1603911	+	363	WP_150749327.1	hypothetical protein	-
PspS49_RS07065	1603988..1605247	-	1260	WP_127926174.1	type II toxin-antitoxin system HipA family toxin	-
PspS49_RS07070	1605240..1605557	-	318	WP_127930362.1	helix-turn-helix transcriptional regulator	-
PspS49_RS07075	1605701..1606108	-	408	WP_137218087.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PspS49_RS07080	1606143..1606298	-	156	WP_150773830.1	type II toxin-antitoxin system HicA family toxin	Toxin
PspS49_RS07090	1606809..1607693	-	885	WP_064119706.1	alpha/beta hydrolase	-
PspS49_RS07095	1608010..1608702	-	693	WP_160038649.1	pseudouridine synthase	-
PspS49_RS07100	1608736..1608966	-	231	WP_127926178.1	cysteine-rich CWC family protein	-
PspS49_RS07105	1608959..1610446	-	1488	WP_127926179.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5826.89 Da
• Isoelectric Point: 11.2584

>T72445 WP_150773830.1 NZ_CP019432:c1606298-1606143 [Pseudomonas sp. S49]
VNSRFLIGQLVAYGWYLVRVRGSHHHFKHPTKPGLVTVPHPTKDLLKQALL

Nucleotide

• Length: 156 bp

>T72445 NZ_CP019432:c1606298-1606143 [Pseudomonas sp. S49]
GTGAATAGCCGTTTTTTAATCGGTCAACTTGTTGCATACGGTTGGTATTTAGTGAGAGTCAGAGGCAGTCACCATCATTT
CAAGCATCCGACCAAGCCTGGACTGGTCACGGTGCCGCATCCGACGAAAGATCTGTTGAAGCAAGCGCTGCTTTGA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14416.43 Da
• Isoelectric Point: 4.4904

>AT72445 WP_137218087.1 NZ_CP019432:c1606108-1605701 [Pseudomonas sp. S49]
MLYPIAISIGDEEHAWGVEVPDIPGCFSAGDDLDDAVAMAREAIEGHFEILAEDGAPIPAASKLGVNVANPKYAGCAWAV
VDIDVVKYLGKAQKLNITLPGHLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT72445 NZ_CP019432:c1606108-1605701 [Pseudomonas sp. S49]
ATGCTCTACCCGATTGCGATTTCAATAGGTGATGAGGAACACGCCTGGGGCGTGGAGGTGCCGGACATTCCGGGCTGTTT
TTCGGCTGGCGATGATCTGGATGATGCCGTCGCCATGGCTCGCGAAGCCATCGAGGGGCATTTCGAAATTTTGGCTGAGG
ACGGTGCGCCGATCCCGGCTGCGAGCAAACTCGGCGTGAACGTCGCCAATCCGAAGTACGCAGGATGTGCATGGGCCGTG
GTCGATATAGATGTCGTGAAGTACTTGGGCAAAGCCCAGAAACTCAACATCACTTTGCCGGGGCACTTGCTCAACCGCAT
TGATGAATATGTGTTGCATCACCCGGAAGAAAAAAGCCGCTCGGGCTTTCTGGCGTCGGCGGCTCTGAAAGTTTTGCAGC
AGGGTTGA