Detailed information of TA system TA072443

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 289528..290171
• Replicon: chromosome
• Accession: NZ_CP019432
• Organism: Pseudomonas sp. S49

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4R7VCK5
• Locus tag: PspS49_RS01255
• Protein ID: WP_026000492.1
• Coordinates: 289528..289710 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PspS49_RS01260
• Protein ID: WP_174245096.1
• Coordinates: 289740..290171 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspS49_RS01240	285527..286453	+	927	WP_016983653.1	serine acetyltransferase	-
PspS49_RS01245	286894..288855	+	1962	WP_174245095.1	choline transporter BetT	-
PspS49_RS01250	288879..289139	-	261	WP_095122232.1	hypothetical protein	-
PspS49_RS01255	289528..289710	+	183	WP_026000492.1	type II toxin-antitoxin system HicA family toxin	Toxin
PspS49_RS01260	289740..290171	+	432	WP_174245096.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PspS49_RS01265	290177..291214	-	1038	WP_160037822.1	LacI family DNA-binding transcriptional regulator	-
PspS49_RS01270	291420..293645	+	2226	WP_160037824.1	TonB-dependent receptor	-
PspS49_RS01275	293648..294967	+	1320	WP_160037826.1	LLM class flavin-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (13-137)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6903.02 Da
• Isoelectric Point: 10.5417

>T72443 WP_026000492.1 NZ_CP019432:289528-289710 [Pseudomonas sp. S49]
MRSREMIRMIEDDGWYLVAVKGSHHQYKHPHKPGRVTIKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T72443 NZ_CP019432:289528-289710 [Pseudomonas sp. S49]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAAGACGATGGTTGGTATCTCGTGGCGGTGAAAGGCAGCCACCACCAATA
CAAGCATCCGCACAAGCCAGGAAGGGTGACGATCAAGCACCCTGATTCGGATCTGCCCAAGGGCACGATCAACAGCATTT
TGAAACAGGCGGGCTTGAAATGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15723.63 Da
• Isoelectric Point: 4.4225

>AT72443 WP_174245096.1 NZ_CP019432:289740-290171 [Pseudomonas sp. S49]
MGLSEEGLTHMKFPVVLHKDADSDYGVIIPDVPGCFSAGTTVAEAFENTQEALALHYEGLVADSAPLPQVRDIDAHLDNP
DYAGGIWGVVDFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYRSRSGFLAAAALRELSV

Nucleotide

• Length: 432 bp

>AT72443 NZ_CP019432:289740-290171 [Pseudomonas sp. S49]
ATGGGCCTGTCAGAGGAAGGACTCACTCATATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCAGACTACGGAGT
GATCATCCCGGATGTCCCGGGTTGTTTCTCCGCAGGCACTACGGTCGCTGAGGCGTTTGAAAATACTCAGGAGGCATTGG
CGCTTCACTATGAGGGGTTGGTCGCCGACAGCGCGCCGTTACCGCAAGTGCGGGACATTGATGCTCATCTCGATAACCCG
GATTACGCGGGAGGGATCTGGGGCGTGGTCGATTTTGATATCACCCCGTATTTCGGCAAGTCAGTGCGTTTTAACGCCAC
GCTGCCGGAGCAGTTGCTTGAGCGTATTGACCAGACAGTCCGCCGTGACCAGCGCTATCGATCCCGTTCCGGATTTCTCG
CGGCGGCCGCCTTGCGCGAACTGTCGGTATAG

Structures

Toxin

• AlphaFold DB: A0A4R7VCK5