Detailed information of TA system TA072442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 5435087..5435673
• Replicon: chromosome
• Accession: NZ_CP019431
• Organism: Pseudomonas sp. S35

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6I6W8S7
• Locus tag: PspS35_RS24455
• Protein ID: WP_056788882.1
• Coordinates: 5435087..5435395 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PspS35_RS24460
• Protein ID: WP_159937129.1
• Coordinates: 5435392..5435673 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspS35_RS24425	5430606..5431226	+	621	WP_159937124.1	HEPN domain-containing protein	-
PspS35_RS24430	5431228..5432298	+	1071	WP_159937125.1	hypothetical protein	-
PspS35_RS24435	5432366..5432800	+	435	WP_057021762.1	hypothetical protein	-
PspS35_RS24440	5432811..5433332	+	522	WP_159937126.1	hypothetical protein	-
PspS35_RS24445	5433332..5434084	+	753	WP_159937127.1	hypothetical protein	-
PspS35_RS24450	5434140..5434991	-	852	WP_159937128.1	hypothetical protein	-
PspS35_RS24455	5435087..5435395	+	309	WP_056788882.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PspS35_RS24460	5435392..5435673	+	282	WP_159937129.1	putative addiction module antidote protein	Antitoxin
PspS35_RS24465	5435757..5436542	-	786	WP_159937130.1	hypothetical protein	-
PspS35_RS24470	5436599..5436919	-	321	WP_159937131.1	hypothetical protein	-
PspS35_RS24475	5437006..5437569	-	564	WP_159937132.1	hypothetical protein	-
PspS35_RS24480	5437641..5438333	-	693	WP_159937133.1	SOS response-associated peptidase	-
PspS35_RS24485	5438350..5438592	-	243	WP_159937134.1	hypothetical protein	-
PspS35_RS24490	5439126..5439473	-	348	WP_202982138.1	DNA binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5384478..5469283	84805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-89)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11478.19 Da
• Isoelectric Point: 7.8182

>T72442 WP_056788882.1 NZ_CP019431:5435087-5435395 [Pseudomonas sp. S35]
MNTIITTHEFDNWLKGLKDVQARLRIVSRILSAEAGNFGDCEPVGEGISEMRIFVGPGYRVYYTRRGEVVYLLLCGGNKS
SQKRDIKLAKSILDEIEQGDVK

Nucleotide

• Length: 309 bp

>T72442 NZ_CP019431:5435087-5435395 [Pseudomonas sp. S35]
ATGAACACAATCATCACTACGCATGAATTTGATAACTGGCTGAAAGGTCTGAAAGACGTACAGGCACGTCTACGGATCGT
TTCTCGGATCTTGAGTGCAGAGGCGGGTAATTTTGGTGACTGTGAGCCGGTGGGCGAAGGCATAAGCGAAATGCGGATCT
TCGTCGGGCCGGGATACCGTGTGTACTACACGCGCCGTGGCGAGGTCGTTTACCTGCTACTGTGTGGCGGCAATAAGTCA
TCACAGAAACGAGACATCAAGCTTGCCAAATCCATCCTCGATGAGATCGAGCAAGGAGACGTAAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10333.87 Da
• Isoelectric Point: 7.0106

>AT72442 WP_159937129.1 NZ_CP019431:5435392-5435673 [Pseudomonas sp. S35]
MRELKAFDASQYLTNEDEIAQFLTISLEDPNPDVFLRALQEVAKARGMSQLARDAGMNRESLYKALAPGAKPRYDTVLKL
VNALGVKLTAQHV

Nucleotide

• Length: 282 bp

>AT72442 NZ_CP019431:5435392-5435673 [Pseudomonas sp. S35]
ATGAGAGAACTCAAAGCATTTGATGCATCCCAATACCTCACCAACGAGGACGAGATTGCTCAATTCCTGACCATTTCTTT
GGAAGACCCAAACCCTGACGTGTTCCTTCGCGCCTTGCAAGAAGTTGCCAAGGCCCGTGGTATGTCGCAGTTGGCCCGTG
ATGCAGGCATGAACCGTGAGAGCCTGTACAAAGCGTTGGCACCAGGTGCAAAGCCCCGGTATGACACAGTGTTGAAGCTC
GTGAATGCATTAGGGGTGAAGCTGACGGCACAGCACGTTTAG

Structures

Toxin

• AlphaFold DB: A0A6I6W8S7