Detailed information of TA system TA072441

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5073141..5073769
• Replicon: chromosome
• Accession: NZ_CP019431
• Organism: Pseudomonas sp. S35

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A6I6VXI6
• Locus tag: PspS35_RS22715
• Protein ID: WP_069558626.1
• Coordinates: 5073141..5073539 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: PspS35_RS22720
• Protein ID: WP_034135537.1
• Coordinates: 5073539..5073769 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspS35_RS22695	5070063..5070332	+	270	WP_159938106.1	DUF2790 domain-containing protein	-
PspS35_RS22700	5070807..5072057	+	1251	WP_032879817.1	ribonucleotide-diphosphate reductase subunit beta	-
PspS35_RS22705	5072120..5072293	+	174	Protein_4474	HNH endonuclease	-
PspS35_RS22710	5072365..5073012	+	648	WP_159938107.1	HAD-IB family hydrolase	-
PspS35_RS22715	5073141..5073539	-	399	WP_069558626.1	tRNA(fMet)-specific endonuclease VapC	Toxin
PspS35_RS22720	5073539..5073769	-	231	WP_034135537.1	antitoxin	Antitoxin
PspS35_RS22725	5074006..5074365	+	360	Protein_4478	hypothetical protein	-
PspS35_RS22730	5074420..5075367	+	948	WP_159936876.1	hypothetical protein	-
PspS35_RS22735	5075474..5075818	+	345	WP_159936877.1	helix-turn-helix transcriptional regulator	-
PspS35_RS22740	5075823..5077325	-	1503	WP_159936878.1	amidase	-
PspS35_RS22745	5077455..5077916	-	462	WP_159938108.1	hypothetical protein	-
PspS35_RS22750	5078117..5078320	+	204	WP_159936879.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14996.24 Da
• Isoelectric Point: 8.0459

>T72441 WP_069558626.1 NZ_CP019431:c5073539-5073141 [Pseudomonas sp. S35]
MLKFMLDTNICIFTIKNRPQQVRETFKKHYGQLAISTVTLMELIYGAEKSPNPERNLADVEGFAARLEVLKYDQDAAMHT
GQLRAELARAGTPIGSYDQMIAGHARSQGLIMVTNNRREFDRVPGLRVEDWV

Nucleotide

• Length: 399 bp

>T72441 NZ_CP019431:c5073539-5073141 [Pseudomonas sp. S35]
ATGCTCAAGTTCATGCTCGATACCAACATCTGTATCTTCACAATCAAAAACAGACCCCAGCAGGTGCGCGAAACATTCAA
AAAGCACTATGGCCAACTAGCTATCAGTACAGTAACCCTGATGGAGCTGATTTACGGCGCGGAAAAATCACCTAACCCAG
AGCGAAACCTGGCTGATGTCGAAGGCTTTGCAGCACGTCTGGAAGTTTTGAAATACGACCAGGATGCCGCTATGCACACA
GGCCAACTTCGTGCTGAGCTAGCGAGAGCTGGTACTCCGATAGGTTCATACGACCAGATGATCGCTGGGCATGCCCGTTC
GCAGGGCCTGATTATGGTCACTAACAACCGGCGCGAGTTCGACCGCGTGCCAGGTTTGCGGGTAGAAGATTGGGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8467.28 Da
• Isoelectric Point: 4.2404

>AT72441 WP_034135537.1 NZ_CP019431:c5073769-5073539 [Pseudomonas sp. S35]
MDQGSVFQSNRSQAVRLPKAAALPDDVKRVDVVSIGRARIITPAGESWDSWFDGEGVTPDFMNDRDQPADQERESF

Nucleotide

• Length: 231 bp

>AT72441 NZ_CP019431:c5073769-5073539 [Pseudomonas sp. S35]
ATGGACCAAGGTTCTGTATTTCAAAGCAACCGCAGCCAAGCTGTACGTTTGCCAAAGGCTGCTGCGCTGCCGGATGACGT
AAAACGTGTAGATGTCGTCTCAATCGGTAGGGCCCGAATCATCACCCCAGCCGGTGAGTCATGGGACAGCTGGTTCGATG
GCGAGGGTGTCACGCCGGACTTCATGAACGACCGGGATCAGCCTGCTGACCAAGAGCGGGAATCTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A6I6VXI6