Detailed information of TA system TA072437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 6161542..6162181
• Replicon: chromosome
• Accession: NZ_CP019428
• Organism: Pseudomonas sp. R76

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PspR76_RS27950
• Protein ID: WP_159960386.1
• Coordinates: 6161999..6162181 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PspR76_RS27945
• Protein ID: WP_159960384.1
• Coordinates: 6161542..6161943 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspR76_RS27935	6157656..6161399	+	3744	WP_159960382.1	EAL domain-containing protein	-
PspR76_RS27945	6161542..6161943	-	402	WP_159960384.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PspR76_RS27950	6161999..6162181	-	183	WP_159960386.1	type II toxin-antitoxin system HicA family toxin	Toxin
PspR76_RS27955	6162692..6163138	+	447	WP_078734923.1	Lrp/AsnC family transcriptional regulator	-
PspR76_RS27960	6163306..6164988	+	1683	WP_159961651.1	NAD(P)/FAD-dependent oxidoreductase	-
PspR76_RS27965	6164999..6165793	+	795	WP_159960388.1	carbon-nitrogen hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6815.91 Da
• Isoelectric Point: 11.1367

>T72437 WP_159960386.1 NZ_CP019428:c6162181-6161999 [Pseudomonas sp. R76]
MRSRELIERLVADGWFEVAVKGSHHQFKHPEKRGRVTVPHPKSEIAKGTLHSIFKSAGLK

Nucleotide

• Length: 183 bp

>T72437 NZ_CP019428:c6162181-6161999 [Pseudomonas sp. R76]
ATGAGAAGCAGAGAGCTGATTGAACGCTTGGTTGCAGATGGATGGTTTGAGGTGGCAGTAAAGGGCAGTCATCACCAGTT
CAAGCACCCTGAGAAGCGGGGACGTGTCACCGTGCCTCACCCCAAGTCAGAGATTGCCAAGGGTACCTTACACAGTATTT
TCAAATCAGCAGGTCTCAAGTGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14637.40 Da
• Isoelectric Point: 4.7378

>AT72437 WP_159960384.1 NZ_CP019428:c6161943-6161542 [Pseudomonas sp. R76]
MKFPVVVHKDADSDYSVTVPDVPGCFSAGSSFSEALDNVREALTLHLEGLVADQEKLPQAQEVDAHLNNPDFAGGVWAIV
DFDLTPYLGKSVRFNASLPEQLLHRIDERVQKDHRYASRSGFLATAALRELSV

Nucleotide

• Length: 402 bp

>AT72437 NZ_CP019428:c6161943-6161542 [Pseudomonas sp. R76]
ATGAAATTCCCAGTGGTAGTACATAAAGACGCCGATTCGGACTACAGCGTGACGGTCCCTGATGTACCTGGTTGCTTTTC
GGCAGGCTCCTCGTTTTCCGAAGCGTTGGATAATGTTCGGGAGGCGTTGACGCTGCACCTGGAAGGGCTGGTTGCCGACC
AGGAAAAACTTCCCCAAGCACAAGAGGTCGATGCTCACTTGAACAATCCGGACTTTGCCGGCGGTGTCTGGGCGATAGTC
GACTTTGATCTCACGCCGTACTTGGGTAAGTCGGTGCGCTTCAATGCCTCTTTGCCGGAACAGTTGCTGCACCGCATTGA
TGAGCGTGTGCAGAAGGATCATCGCTATGCATCGCGGTCAGGCTTTTTGGCGACGGCGGCGTTGCGTGAGTTGTCTGTGT
AA