Detailed information of TA system TA072435

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2900470..2901083
• Replicon: chromosome
• Accession: NZ_CP019428
• Organism: Pseudomonas sp. R76

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: PspR76_RS13295
• Protein ID: WP_159955883.1
• Coordinates: 2900832..2901083 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PspR76_RS13290
• Protein ID: WP_159955881.1
• Coordinates: 2900470..2900835 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PspR76_RS13265	2895656..2897011	+	1356	WP_159955871.1	MATE family efflux transporter	-
PspR76_RS13270	2897012..2897473	-	462	WP_159955873.1	DUF3225 domain-containing protein	-
PspR76_RS13275	2897540..2898505	-	966	WP_159955875.1	GlxA family transcriptional regulator	-
PspR76_RS13280	2898547..2899155	-	609	WP_159955877.1	TetR family transcriptional regulator	-
PspR76_RS13285	2899297..2900469	+	1173	WP_159955879.1	MFS transporter	-
PspR76_RS13290	2900470..2900835	-	366	WP_159955881.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PspR76_RS13295	2900832..2901083	-	252	WP_159955883.1	type II toxin-antitoxin system HicA family toxin	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9463.96 Da
• Isoelectric Point: 10.0953

>T72435 WP_159955883.1 NZ_CP019428:c2901083-2900832 [Pseudomonas sp. R76]
VSKNEKLLAKLLNQNTAFTWPELVTLLQRLGYTQIEGAGSRVKFDIGDPSAMITLHRPHPGNELKHYIRRQIIEQLKSGE
LIQ

Nucleotide

• Length: 252 bp

>T72435 NZ_CP019428:c2901083-2900832 [Pseudomonas sp. R76]
GTGTCCAAAAATGAAAAGCTGCTCGCCAAACTGCTCAACCAGAACACGGCCTTTACCTGGCCAGAGCTCGTAACGCTGTT
GCAGCGTCTTGGCTACACACAGATTGAAGGGGCCGGAAGCCGCGTCAAATTCGACATCGGCGACCCCAGTGCAATGATCA
CCTTGCACAGGCCTCACCCCGGCAACGAACTGAAACACTACATTCGGCGCCAGATCATCGAACAATTGAAATCAGGAGAA
CTGATTCAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13249.11 Da
• Isoelectric Point: 6.7402

>AT72435 WP_159955881.1 NZ_CP019428:c2900835-2900470 [Pseudomonas sp. R76]
VNNQLKHNGYIGSIEASLEDNCLFGKVLFIKALVSYEGKTVAELDVAFREAVDDYLATCHTLGQTPEKPCKGSFNVRVGH
DLHLAAALAATRKKVTLNDLTRQALNEFLQHHCAEPFPAIG

Nucleotide

• Length: 366 bp

>AT72435 NZ_CP019428:c2900835-2900470 [Pseudomonas sp. R76]
GTGAACAATCAACTGAAACACAATGGCTACATCGGCTCTATCGAAGCCAGCCTCGAAGACAACTGCCTGTTCGGCAAAGT
GCTGTTTATCAAGGCACTGGTCAGCTATGAAGGCAAAACCGTCGCGGAGCTGGACGTGGCGTTTCGGGAGGCCGTGGATG
ACTATCTCGCTACGTGCCACACCCTCGGGCAAACACCGGAGAAACCGTGCAAGGGTTCATTCAACGTGCGGGTCGGCCAT
GACCTGCATCTGGCGGCAGCCCTGGCAGCGACCCGTAAGAAAGTGACGCTCAATGACCTGACACGCCAGGCGCTGAATGA
GTTTTTGCAACATCATTGCGCAGAACCTTTCCCGGCGATCGGCTGA